Anti grhl2

Anti-FOXA1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA, catalogue no. sc-101058) used at 1:250 for IHC and 1:400 for immunoblot. Anti-CTCF (Cell Signaling, Beverley, MA, USA, catalogue no. 2899) used at 1:250 for IHC and 1:1000 for immunoblot. Anti-P63 (Santa Cruz Biotechnologies, catalogue no. sc-8431) used at 1:1000 for IHC and 1:500 for immunoblot. Anti-GRHL2 (Abcam, catalogue no. ab88631) used at 1:150 for IHC and 1:400 for immunoblot. Anti-PPARγ (Santa Cruz, catalogue no. 7273) used at 1:2000 for IHC and 1:500 for immunoblot. Anti-GATA3 (Cell Signalling, catalogue no. 5852) used at 1:800 for IHC and 1:1000 for immunoblot. Anti-GRHL3 (Abcam, Cambridge, UK, catalogue no. ab57612) used at 1:500 for immunoblot. Anti-KRT13 (Abnova, Taipei City, Taiwan, catalogue no. MAB1864) used at 1:1000 for immunoblot. Anti-BACT (Sigma-Aldrich, St. Louis, MO, USA, catalogue no. AC5441) used at 1:250 000 for immunoblot).

The lentiviral-Grhl2-puro and lentiviral-control-puro were purchased from SunBio (Shanghai, China). Anti-Grhl2 was purchased from Abcam Biotechnology (Cambridge, UK). Anti-MMP-2, MMP-7, MMP-9, SMAD2 and β-actin were from Proteintech Group Inc. (Wuhan, China). Anti-E-cadherin was purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-Vimentin, TGFβR1 and N-cadherin were obtained from Epitomics Inc. (Burlingame, CA, USA). LY364947 and TGFβ1 were purchased from Sigma-Aldrich (St Louis, MO, USA).