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Micromass detector

Manufactured by Waters Corporation
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The MICROMASS detector is a mass spectrometry instrument designed for analytical applications. It is capable of detecting and measuring the mass-to-charge ratio of ionized molecules and atoms. The MICROMASS detector can be used to identify and quantify a wide range of chemical compounds in various samples.

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Lab products found in correlation

Acquity ultra performance lc, by Waters Corporation (7 mentions) 1k ccd camera, by Ametek (2 mentions) Morgagni 268 tem, by Ametek (2 mentions) Lsm 880, by Zeiss (2 mentions) Unity inova 400, by Agilent Technologies (1 mentions) Delta 600 hplc system, by Waters Corporation (1 mentions) Rp c18 column, by Waters Corporation (1 mentions) Fmoc amino acids, by GL Biochem (1 mentions) 2 cl trityl chloride resin, by GL Biochem (1 mentions) Unity inova 400 spectrometer, by Agilent Technologies (1 mentions) Rf 5301pc fluorescence spectrophotometer, by Shimadzu (1 mentions)

7 protocols using micromass detector

1

Multi-Modal Analysis of Bio-Samples

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LC-MS on Waters Acquity ultra Performance LC with Waters MICROMASS detector, rheological measurement on ARES-G2 rheometer; electron microscopy was performed on a FEI Morgagni 268 TEM with a 1k CCD camera (GATAN, Inc., Pleasanton, CA); MTT assay for cell toxicity test on DTX880 Multimode Detector.
Shi J., Du X., Yuan D., Zhou J., Zhou N., Huang Y, & Xu B. (2014). d-Amino Acids Modulate the Cellular Response of Enzymatic-Instructed Supramolecular Nanofibers of Small Peptides. Biomacromolecules, 15(10), 3559-3568.
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2

Purification and Characterization of Product

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We used Waters Delta600 HPLC system, which equipped with an XTerra C18 RP column and an in-line diode array UV detector to purify the product. LC-MS machine we used was a Waters Acquity Ultra Performance LC with Waters MICROMASS detector. Hydrogen nuclear magnetic resonance (NMR) spectra were recorded on a Varian Unity Inova 400 with DMSO as solvent. We used Morgagni 268 transmission electron microscope to take Transmission electron microscope (TEM) images. MTT assay for cell cytotoxicity was test on DTX880 Multimode Detector.
Li J., Du X., Powell D.J., Zhou R., Shi J., He H., Feng Z, & Xu B. (2018). Down-regulating Proteolysis to Enhance Anticancer Activity of Peptide Nanofibers. Chemistry, an Asian journal, 13(22), 3464-3468.
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3

Fluorescent Amino Acid Labeling

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All amino acids were purchased from GL Biochem, and NBD-Cl was purchased from TCI America. All the solvents and chemical reagents were used directly as received from the commercial sources without further purification. All the products were purified with HPLC system (Agilent 1100 Series). Confocal microscopy images were obtained on by ZEISS LSM 880 confocal laser scanning microscope. Electron microscopy imaging was performed on an Morgagni 268 transmission electron microscrope. LC-MS was operated on a Waters Acquity Ultra Performance LC with Waters MICRO-MASS detector.
Yang D., Kim B.J., He H, & Xu B. (2020). Enzymatically Forming Cell Compatible Supramolecular Assemblies of Tryptophan-Rich Short Peptides. Peptide science (Hoboken, N.J.), 113(2), e24173.
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4

Synthesis and Characterization of Peptides

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2-Cl-trityl chloride resin (0.8–1.0 mmol/g), HBTU and Fmoc-amino acids were purchased from GL Biochem (Shanghai, China). Other chemical reagents and solvents were obtained from Fisher Scientific. We synthesized peptide 1-EP, 1-CP, and 1-E by combining solid phase peptide synthesis (SPPS) and solution-phase synthesis (Scheme S2). After the synthesis, all compounds were purified using a reverse phase HPLC (Agilent 1100 Series) with HPLC grade acetonitrile (0.1% TFA) and HPLC grade water (0.1% TFA) as the eluents. The 1H-NMR and 31P-NMR spectra of compounds were obtained using Varian 400 MR and LC-MS spectra using a Waters Acquity Ultra Performance LC with Waters MICROMASS detector.
Feng Z., Wang H., Yi M., Lo C.Y., Sallee A., Hsieh J.T, & Xu B. (2019). Instructed-Assembly of Small Peptides Inhibits Drug-Resistant Prostate Cancer Cells. Peptide science (Hoboken, N.J.), 112(1), 10.1002/pep2.24123.
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5

Characterization of Nanoparticle Conjugates

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Conjugates were purified with a Water Delta600 HPLC system, equipped with an XTerra C18 RP column and an in-line diode array UV detector. 1H NMR spectra were obtained on a Varian Unity Inova 400 spectrometer. LC–MS spectra were performed on Waters Acquity Ultra Performance LC with Waters MICROMASS detector. TEM images were taken on a Morgagni 268 transmission electron microscope. Rheological data were measured on a TA ARES G2 rheometer with 25 mm cone plate. MTT assay for cell toxicity test were measured on a DTX880 Multimode Detector.
Yuan D., Du X., Shi J., Zhou N., Baoum A.A., Al Footy K.O., Badahdah K.O, & Xu B. (2015). Synthesis and evaluation of the biostability and cell compatibility of novel conjugates of nucleobase, peptidic epitope, and saccharide. Beilstein Journal of Organic Chemistry, 11, 1352-1359.
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6

Analytical Techniques for Peptide Characterization

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All peptides were purified by Water Agilent 1100 HPLC system, equipped with an XTerra C18 RP column. LC-MS was operated on a Waters Acquity Ultra Performance LC with Waters MICRO-MASS detector. Transmission electron microscope (TEM) images were taken on Morgagni 268 transmission electron microscope. Fluorescent analysis was performed on Shimadzu RF-5301-PC fluorescence spectrophotometer. Fluorescence images were taken by ZEISS LSM 880 confocal laser scanning microscope.
He H., Lin X., Guo J., Wang J, & Xu B. (2020). Perimitochondrial Enzymatic Self-Assembly for Selective Targeting the Mitochondria of Cancer Cells. ACS nano, 14(6), 6947-6955.
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7

Comprehensive Multi-Analytical Characterization

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LC-MS
on Waters Acquity
ultra Performance LC with Waters MICROMASS detector, rheological measurement
on ARES-G2 rheometer; electron microscopy was performed on a FEI Morgagni
268 TEM with a 1k CCD camera (GATAN, Inc., Pleasanton, CA); MTT assay
for cell toxicity test on DTX880 Multimode Detector.
Shi J., Du X., Yuan D., Zhou J., Zhou N., Huang Y, & Xu B. (2014). d-Amino Acids Modulate the Cellular Response of Enzymatic-Instructed Supramolecular Nanofibers of Small Peptides. Biomacromolecules, 15(10), 3559-3568.
+ Open protocol
+ Expand

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