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4 protocols using methylene blue

1

Expanded PS Polymer Recycling and Characterization

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Expanded (PS), a commercial recycled polymer, Polybutylene succinate (PBS), 50% bio-sourced, (reference PBE 003 BB) was supplied by Natureplast© (Ifs, France). Sulfuric acid was a product of ADWIC Co (Egypt). Toluene and Chloroform (> 99%) were obtained from Fisher (United Kingdom), 1,4-Dioxan (> 99.5%) was purchased from Elnasr pharmaceutical chemicals co. (Egypt). Lithium chloride was obtained from Sigma-Aldrich (> 99%). Methylene blue (Fig. 1d) was purchased from Sd Fine-Chem Limited (India).

Chemical structure of (a) polystyrene (PS), (b) polystyrene sulfonate (PSS), (c) Polybutylene succinate (PBS), and (d) Methylene blue dye.

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2

Chitin-based Photocatalytic Nanomaterials

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Cadmium Oxide (CdO), Titanium Tetra IsoPropoxide (TTIP), Tween-80, Ethanol and Isopropyl alcohol (IPA) were procured from Merck. Crab shells (Chitin) were purchased at Seafood Market, Kasimedu, Chennai, India. Methyl orange, Methylene blue, and Rhodamine B were supplied by S.D. Fine Chemicals, India.
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Acyclovir Topical Formulation Development

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Acyclovir was a kind gift from Zen Lab & Preet Remedies Pvt. Ltd., Baddi, India.
Cellulose acetate 398-10 was brought from Sigma Aldrich Chemical, Germany; glycerol was brought from S. D. Fine Chemicals, Mumbai, India; sodium lauryl sulphate was brought from Ranbaxy Fine Chemicals Ltd., New Delhi, India. Acetone, ethanol 95% v/v, potassium dihydrogen orthophosphate, and methylene blue were obtained from S. D. Fine Chemicals, Mumbai, India.
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4

Synthesis of Photocatalytic Nanoparticles

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Titanium iso-propoxide (>97% purity) was purchased from Sigma-Aldrich. L-Ascorbic acid-LR, silver nitrate, di-sodium hydrogen phosphate and methylene blue, methyl orange, neutral red dyes were purchased from SD Fine Chemicals Ltd (India). Sodium hydroxide, sulphuric acid (98%), hydrochloric acid (35%) and nitric acid (69%) were purchased from Merck (India). The Neubauer-chamber was purchased from Pristine Scientific Ltd (India). Luria nutrient broth was purchased from Hi Media (India). Double distilled Millipore water was used for all the experiments.
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