Stream basic

Manufactured by Olympus

Sourced in Japan

The Stream Basic is a laboratory equipment product designed for general-purpose scientific applications. It provides fundamental functionality for data acquisition and analysis. The core function of the Stream Basic is to enable the collection and processing of experimental data.

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Lab products found in correlation

Bx53m, by Olympus (2 mentions) Bond max, by Leica (1 mentions) Axio zoom v16 stereomicroscope, by Zeiss (1 mentions) Sc100, by Olympus (1 mentions) Szx16 stereomicroscope, by Olympus (1 mentions) Eos 5d mark 2, by Zeiss (1 mentions) Bx51 microscope, by Olympus (1 mentions) Uc30 digital camera, by Olympus (1 mentions)

Close spelling variants of this product

Stream Basic software (6 citations) Stream Basic imaging software (3 citations) Stream Basic 1.9 software (2 citations)

7 protocols using stream basic

Optical Microscopy Examination of 5MeODMT Benzoate

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Example 20

Optical microscopy examination was undertaken using an Olympus BX53M polarised light microscope and an Olympus SC50 digital video camera for image capture using imaging software Olympus Stream Basic, V2.4. The image scale bar was verified against an external graticule, 1.5/0.6/0.01 mm DIV, on a monthly basis.

A small amount of each sample was placed onto a glass slide and dispersed using mineral dispersion oil if required. The samples were viewed with appropriate magnification and various images recorded.

Optical micrographs of 5MeODMT benzoate salt, were acquired. The material is composed of large rhombohedral/trigonal crystals, ranging from 400 to 1000 microns. There are also small crystals adhering to the large crystals. Some of the small crystals, from 10 microns, are a consequence of mechanical attrition, but others have formed by crystallisation. There are also large aggregates composed of various habits. FIGS. 13 to 16 show various optical micrographs of 5MeODMT benzoate at various magnifications.

US11603353B2. Composition comprising a benzoate salt of 5-methoxy-N,N-dimethyltryptamine (2023-03-14). Beckley Psytech Limited [GB]. Inventors: Cosmo Feilding-Mellen [GB], Timothy Mason [GB].

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Fiber Morphology and Density Analysis

Cited 1 time

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Morphology and geometry of fibers were evaluated by the counting method using software Olympus Stream Basic [25 (link)]. The average diameter of fibers was determined manually for each fiber on the z-stack at five different points of the sample excluding defective areas.
Average density characterizes the mass per unit volume of the material. The data were averaged over ten samples. The density,

δ

, was defined as:

δ = \frac{m}{l \times B \times b}

where m is the mass; l is the length; B is the width; and b is the thickness.
Theoretical porosity is the percentage of the mass of the material and the fiber-free volume. The data were averaged over five samples.

Tyubaeva P., Varyan I., Lobanov A., Olkhov A, & Popov A. (2021). Effect of the Hemin Molecular Complexes on the Structure and Properties of the Composite Electrospun Materials Based on Poly(3-hydroxybutyrate). Polymers, 13(22), 4024.

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Characterizing Fibrous Material Structure

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The structure of fibrous materials was evaluated by the counting method using the software Olympus Stream Basic (Tokyo, Japan). PHB-Fe(TPP)Cl and PHB-Hmi samples 100 × 100 mm were used for the counting method.
The average diameter of fibers was determined manually for each fiber on the z-stack at 10 different points of the sample excluding defective areas.
Average density characterizes the mass per unit volume of the material. The data were averaged over 10 samples. The density, δ, was defined as:

δ = \frac{m}{l x B x b},

Tyubaeva P., Varyan I., Krivandin A., Shatalova O., Karpova S., Lobanov A., Olkhov A, & Popov A. (2022). The Comparison of Advanced Electrospun Materials Based on Poly(-3-hydroxybutyrate) with Natural and Synthetic Additives. Journal of Functional Biomaterials, 13(1), 23.

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Histological Assessment of Skin Angiogenesis

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At the end of the experimental period, the animals were sacrificed and skin samples were harvested for histological examination. Skin samples were fixed in 10% buffered neutral formalin, embedded in paraffin, sections were made at 4 micrometers and the slides were stained by Haematoxiline–Eosine (HE) method. The slides were examined under a microscope Olympus BX 51. The images were taken with Olympus UC 30 digital camera and processed by a special image acquisition and processing program: Olympus Stream Basic. The angiogenesis was assessed by α-SMA immunostaining using a mouse monoclonal antiα-SMA antibody (clone 1A4) (Abcam ab76549) in a 1:800 dilution. The reaction was carried by a Leica Bond-Max (Leica Microsystems, Wetzlar, Germany) automated immunostainer using a polymer-based detection system (Leica Biosystems, Wetzlar, Germany) with 3,3′-Diaminobenzidinew (DAB) as the chromogen. Masson-Goldner’s trichrome (TM)-stained method was used for histological examination during the wound healing process (day 8).

Mârza S.M., Magyari K., Bogdan S., Moldovan M., Peștean C., Nagy A., Gal A.F., Tăbăran F., Purdoiu R.C., Licărete E., Suarasan S., Baia L, & Papuc I. (2021). The Impact of Composites with Silicate-Based Glasses and Gold Nanoparticles on Skin Wound Regeneration. Molecules, 26(3), 620.

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Optical Microscopy Examination of 5MeODMT Benzoate

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Example 20

A small amount of each sample was placed onto a glass slide and dispersed using mineral dispersion oil if required. The samples were viewed with appropriate magnification and various images recorded.

US11518742B2. Composition comprising a benzoate salt of 5-methoxy-N,N-dimethyltryptamine (2022-12-06). Beckley Psytech Limited [GB]. Inventors: Cosmo Feilding-Mellen [GB], Timothy Mason [GB].

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Aeolian Sand Dune Arthropod Diversity Assessment

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We carried out our research on aeolian sand dunes located in Záhorie Protected Landscape Area, Borská nížina, during 2018 and 2019 (Fig. 1,2A). The study area serves as a military complex used by the Slovak army. Additionally, three males of W. stylifrons were collected in a viticultural landscape near the town of Modra (Fig. 2B). The material was obtained using pitfall traps.
Measurements of W. stylifrons were obtained using an Olympus SC 100 attached to an Olympus SZx16 stereomicroscope and edited with Olympus Stream basic. Measurements of S. mongolica were taken from digital images using the AxioVision 40LE application. Digital photographs were taken using a Canon EOS 5D Mark II camera attached to a Zeiss Axio Zoom V16 stereomicroscope. Image stacks were produced manually, combined using Zerene Stacker software and subsequently edited in Adobe Photoshop CC. Nomenclature follows World Spider Catalog (2021).
The specimens are preserved in 70% ethanol and deposited in the collections of Institute Landscape Ecology SAS in Nitra (ILE SAS, curator: P. Purgat).

Purgat P., Gajdoš P., Purkart A., Hurajtová N., Volnár Ľ., & Krajčovičová K. (2021). Walckenaeria stylifrons and Spiracme mongolica (Araneae, Linyphiidae, Thomisidae), two new species to Slovakia. Check List, 17(6), 1601-1608.

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Histological Examination of Hepatic Samples

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For the histological study, the hepatic samples were fixed in 10% buffered neutral formalin, embedded in paraffin, while sections were made at 4 micrometers and the slides were stained with hematoxylin and eosin (H&E) dye. Then the slides were examined under an Olympus BX 51 microscope. The images were taken with an Olympus UC 30 digital camera and processed by a special image acquisition and processing program, i.e. Olympus Stream Basic.

Chiş I.C., Mureşan A., Oros A., Nagy A.L, & Clichici S. (2016). Protective effects of Quercetin and chronic moderate exercise (training) against oxidative stress in the liver tissue of streptozotocin-induced diabetic rats. Physiology international, 103(1).

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