Female NOD mice were purchased from the Jackson Laboratory (Bar Harbor, ME) at 8 weeks of age and were housed in a specific pathogen-free animal facility until some of them spontaneously developed diabetes. Spines and IVDs were isolated from NOD mice and age-matched female euglycemic mice (19–22 weeks of age) for analyses. DM in NOD mice was confirmed by consecutive blood glucose monitoring (see below). All the experiments were approved by the University of Pittsburgh Institutional Animal Care and Use Committee.
Female nod mice
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
Female NOD mice are a laboratory mouse strain used in biomedical research. They are genetically prone to developing autoimmune diabetes, a condition similar to type 1 diabetes in humans. These mice are commonly used to study the pathogenesis and potential treatments for autoimmune diseases.
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Lab products found in correlation
Contour blood glucose meter, by Bayer (2 mentions)
Female c57bl 6 mice, by Jackson ImmunoResearch (2 mentions)
Onetouch glucometer, by LifeScan (2 mentions)
Ultrasensitive elisa kit, by Mercodia (2 mentions)
Onetouch ultra 2 blood glucose meter, by LifeScan (1 mentions)
Collagenase, by Merck Group (1 mentions)
Recombinant mouse il 1β, by Merck Group (1 mentions)
Hoechst 33342 dye, by Thermo Fisher Scientific (1 mentions)
Recombinant mouse tnf α, by Enzo Life Sciences (1 mentions)
Recombinant mouse ifn γ, by R&D Systems (1 mentions)
Histopaque 1119, by Merck Group (1 mentions)
C57bl 6 mice, by Charles River Laboratories (1 mentions)
13 protocols using female nod mice
1
Spinal Analyses in Diabetic NOD Mice
2
Transgenic Mouse Models for Diabetes Research
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All animal experimental protocols were approved by the Institutional Animal Care and Use Committees of the University of Texas Southwestern Medical Center at Dallas, Dallas Veterans Administration Medical Center, University of Utah, or Vanderbilt University Medical Center (VUMC). Institutional guidelines for animal care and use were followed. With the exception of NOD mice, all mice were bred in-house with constant temperature on 12-h light cycles. PANIC-ATTAC experiments were performed on male or female mice beginning at 9 to 14 wk of age (17 (link), 27 (link)). Female NOD mice (The Jackson Laboratory) were maintained in-house beginning at 8 wk of age. Transgenic mice, with two autosomal copies of the RIP-DTR transgene (22 (link)), in which DTR expression is driven by the RIP, were previously backcrossed onto the NSG background (28 (link)) for more than 10 generations, resulting in the NSG-DTR mouse maintained at VUMC (29 (link)).
3
NOD Mouse Diabetes Progression
4
Murine Model for Cancer Research
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Female C57BL/6 mice were purchased from The Jackson Laboratories. Female NOD mice were purchased from The Jackson Laboratories and bred in-house. Animal studies were conducted following a protocol approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Utah. The group sizes of mice were determined and approved by the regulatory authorities for animal welfare after a balanced consideration of statistical and scientific needs and ethical aspects. All procedures related to animal studies are in compliance with relevant ethical regulations on animal research at the University of Utah. The EL4 and B16-F10 (abbreviated as B16 cells) cell lines were purchased from the ATCC and maintained in DMEM medium with 10% FBS.
5
Monitoring NOD Diabetes Progression
6
NOD mice vaccine against type 1 diabetes
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Female NOD mice were purchased from the Jackson Laboratories (Bar Harbor, ME, USA) and maintained in specific pathogen free facilities at the University of Florida. All animal experimental procedures were conducted in accordance with protocols approved by the University of Florida Institutional Animal Care and Use Committee. A cohort of 8-week old Female NOD mice was divided into two treatment groups (n = 10/group). Experimental groups were as follows: Hydrogel (GM-CSF/CpG) + insulin MPs; Hydrogel (GM-CSF) + empty MPs; and a no treatment control. The vaccine formulations were administered at 8, 10, and 12 weeks of age. Per injection, a total volume of 100 μL was administered, providing the following doses of each component: GM-CSF (40 ng/injection); CpG (10 μg/injection); PLGA MPs (5 mg/injection); 1% hydrogel solution (91 μL/injection). Vaccine formulations were injected at the subcutaneous dorsal neck region. Blood glucose levels were measured once per week with a OneTouch® Ultra® 2 blood glucose meter (Lifescan, Inc., Milpitas, CA, USA). If the value was higher than 250 mg/dL for two consecutive days, the animal was considered to be diabetic and withdrawn from the study.
7
NOD Mice Immunological Studies
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Female NOD mice at 6‐8 weeks of age were purchased from the Jackson Laboratory (Bar Harbor, Maine) and used for the experiments. All mouse studies were approved by the Institutional Animal Care and Use Committee at the Medical University of South Carolina.
8
Murine Model for Cancer Research
9
NOD Mice Diabetes Induction Protocol
10
Isolation and Culture of Mouse Islet Cells
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Chemicals Recombinant mouse IL-1β, hexadimethrine bromide, collagenase and Histopaque 1119 and 1077 were purchased from Sigma-Aldrich (St Louis, MO, USA). Recombinant mouse TNF-α was purchased from Enzo Life Sciences (Farmingdale, NY, USA), and recombinant mouse IFN-γ from R&D Systems (Minneapolis, MN, USA). Hoechst dye 33342 was from Invitrogen (Basel, Switzerland).
Isolation, culture and dissociation of primary islet cells Mouse pancreatic islets were isolated from female NOD mice (Jackson Laboratory, Bar Harbor, ME, USA), male NOD severe combined immunodeficiency (SCID) mice (Janvier Labs, Le Genest-Saint-Isle, France) and C57BL/6 mice (13-14 weeks old; Charles River Laboratories, L'Arbresle, France). All animal procedures were performed in accordance with the National Institutes of Health guidelines and protocols were approved by the Swiss research council and veterinary offices. Mice islets were isolated by collagenase digestion [23] of the pancreas followed by Histopaque density gradient separation. Details about islet handling are provided in electronic supplementary material (ESM) Methods.
Isolation, culture and dissociation of primary islet cells Mouse pancreatic islets were isolated from female NOD mice (Jackson Laboratory, Bar Harbor, ME, USA), male NOD severe combined immunodeficiency (SCID) mice (Janvier Labs, Le Genest-Saint-Isle, France) and C57BL/6 mice (13-14 weeks old; Charles River Laboratories, L'Arbresle, France). All animal procedures were performed in accordance with the National Institutes of Health guidelines and protocols were approved by the Swiss research council and veterinary offices. Mice islets were isolated by collagenase digestion [23] of the pancreas followed by Histopaque density gradient separation. Details about islet handling are provided in electronic supplementary material (ESM) Methods.
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