Rat pheochromocytoma PC12 cell line was purchased from the Shanghai Institute of Biochemistry and Cell Biology (Chinese Academy of Sciences). PC12 cells cultured in PLL-coated 96-well plates were incubated with OABL or OABL-GSH in fresh medium containing 2% horse serum (HS) and 1% FBS for 24 h at 37 °C. After replacement with fresh medium containing 0.6 mM H2O2, 1.0 mM 6-OHDA, 100 mM glutamate (Santa Cruz Biotechnology, U.S.) or 10 μM RSL3 (Sigma, U.S.) for 24 h, MTT (Solarbio, China) was added and incubated for another 4 h. After the solubilization of purple formazan in DMSO, the optical density of absorbance was measured at 490 nm.
6 ohda
Manufactured by Santa Cruz Biotechnology
Sourced in United States
6-OHDA is a neurotoxin primarily used in research applications. It selectively damages dopaminergic and noradrenergic neurons. This product is commonly used to create animal models of Parkinson's disease.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 6 ohda
1
Neuroprotective Potential of OABL and OABL-GSH
2
6-OHDA Induced Neuropathic Pain Model
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For systemic injection, mice received intraperitoneal injections of 6-OHDA (100 mg/kg, Santa Cruz) in saline for 5 consecutive days either at 8 weeks after SNI, or without prior injury. Control mice received intraperitoneal injections of saline. For local injection, under deep isoflurane anesthesia, mice received an injection of 6-OHDA (2 μl, 100 μg/μl) or saline, delivered via a Hamilton syringe connected to a 30G needle, into the hindpaw plantar hairy skin for 5 d.
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