Rabbit anti cxcr4

The whole cell protein was extracted using RIPA buffer combined with protease inhibitor (Merck Millipore). The protein concentration was determined by the Bradford method (Beyotime, Shanghai, China). Protein was separated by 10% SDS/PAGE (Beyotime) and transferred to polyvinylidene fluoride membrane (Merck Millipore). The members were blocked in 5% skim milk with TBST for 1 h at room temperature. Then, the primary antibodies were incubated at 4 °C overnight and secondary antibodies for 1 h at room temperature. The primary antibodies used were mouse anti-GAPDH (Sigma, USA), rabbit anti-CXCR4 and rabbit anti-CXCR12 (Proteintech, Rosemont, IL, USA).

Rabbit anti-SMA and rat anti-MOMA2 polyclonal antibodies were purchased from Santa Cruz Biotechnology (Dallas, Texas, USA). Rabbit anti-CXCR4, GAPDH, RGS16, VCAM-1, and SM22α polyclonal antibodies and mouse anti-CD31 monoclonal antibody were purchased from Proteintech Group (Chicago, IL, USA). Rabbit anti-CXCL12 polyclonal antibody was purchased from Novus Biologicals (USA). U0126 (a MEK1/2 inhibitor) was purchased from LC Laboratories (Woburn, MA, USA). miR-126-3p antagomir and ERK1/2 siRNA were purchased from Guangzhou Ribobio (Guangzhou, Guangdong, China). Triglyceride (TG) kit, Total cholesterol (TC) kit, High-density lipoprotein Cholesterol (HDL-C) kit and Low-density lipoprotein cholesterol (LDL-C) kit were purchased from Medicalsystem Biotechnology Co., Ltd (Ningbo, Zhejiang, China).