Glycerol

Medium-molecular-weight CS 80/50 (degree of deacetylation: 77.6–82.5%, viscosity of 1% solution in 1% acetic acid: 31–70 mPa·s, molecular weight: 80–200 kDa) was obtained from Heppe Medical CS GmbH (Haale, Germany). Low-methoxy amidated PC with low- (a type CF 010, degree of esterification 34%, degree of amidation 17%, pH 4.2 for 2.5% solution in distilled water at 20 °C) and high-calcium reactivity (a type CF 020, degree of esterification 31%, degree of amidation 19%, pH 4.1 for 2.5% solution in distilled water at 20 °C) were kindly gifted by Herbstreith & Fox and GmbH & Co. KG (Neuenbürg, Germany). Potassium dihydrogen phosphate, sodium chloride, calcium chloride dihydrate, and 85% lactic acid were purchased from Chempur (Piekary Śląskie, Poland). Glycerol was obtained from Fagron (Kraków, Poland).
SSS composed of Potassium dihydrogen phosphate (1.63 mg/mL), sodium chloride (2.32 mg/mL), and calcium chloride dihydrate (0.22 mg/mL) with pH 6.2 adjusted by disodium hydrogen phosphate addition (according to Nair et al. with modification [25 (link)]) was used for the research analyses.

Clotrimazol was purchased from Pol-Aura (Poznan, Poland). Carbopol^® EZ-3 was purchased from Lubrizol Corp. (Wickliffe, OH, USA). Glycerol was purchased from Fagron (Kracow, Poland). Ethanol 96%, isopropanol and sodium chloride were purchased from Avantor Performance (Gliwice, Poland). Phosphate buffer concentrate was purchased from Chempur (Piekary Śląskie, Poland). Resin was purchased from Phrozen Aqua-Blue (Hsinchu City, Taiwan). Triizopropanolamine was purchased from Sigma-Aldrich (St. Luis, MI, USA). Sabouraud dextrose and yeast culture broth were purchased from Merck KGaA (Darmstadt, Germany).