Streptavidin allophycocyanin apc

Manufactured by BD

Streptavidin allophycocyanin (APC) is a fluorescent conjugate used in flow cytometry and other bioanalytical applications. It consists of the protein streptavidin coupled to the fluorescent dye allophycocyanin. Streptavidin has a high affinity for the small molecule biotin, allowing it to serve as a detection reagent for biotinylated targets.

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Lab products found in correlation

Streptavidin bv421, by BD (1 mentions) Pierce zeba desalting column, by Thermo Fisher Scientific (1 mentions) D biotin, by Avidity (1 mentions) Anti cd21 fitc, by BD (1 mentions) Anti cd23 pe, by BD (1 mentions) Facscalibur, by BD (1 mentions) Cellquest, by BD (1 mentions) Annexin 5 fitc, by BD (1 mentions) Anti cd19 apc, by BD (1 mentions) Streptavidin pe, by BD (1 mentions) Pe anti igma, by BD (1 mentions) Anti b220 fitc, by BD (1 mentions) Anti b220 percp, by BD (1 mentions) Facscelesta cell analyzer, by BD (1 mentions) 7aad dye, by BD (1 mentions) Biotin conjugated annexin 5, by BD (1 mentions) Annexin 5 binding buffer, by BD (1 mentions)

Spelling variants of this product

Streptavidin-APC (91 citations) Streptavidin-allophycocyanin (12 citations) Allophycocyanin (APC)-conjugated streptavidin (6 citations) Allophycocyanin (APC)-labeled streptavidin (2 citations)

3 protocols using streptavidin allophycocyanin apc

SARS-CoV-2 Spike Protein Biotinylation

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Full‐length SARS‐CoV‐2 Spike (2P‐stabilized, C‐terminal Histidine/Avi‐tagged) was obtained from BEI resources (Manassas, VA, Cat. NR53524) and biotinylated using a BirA ubiquitin ligase (Avidity, Aurora, CO, Cat. Bir500A) following the manufacturer's recommended protocol. Biotinylated spike protein was purified using a 40 K molecular weight cut‐off (MWCO) 2 ml Pierce Zeba™ desalting column (Thermo Fisher Scientific, Waltham, MA, Cat. 87768), and mixed with streptavidin BV421 (BD, Cat. 563259) or streptavidin allophycocyanin (APC) (BD Cat. 554067) separately at a 20:1 ratio (~6:1 molar ratio) as previously described [3 (link)]. Tetramerized Spike probes were stored at 4°C until use. Just prior to probe staining, spike protein probes were added one‐by‐one to FACS wash buffer (1x PBS, 2% fetal bovine serum) containing 5 μM free d‐biotin (Avidity, Cat. Bir500A). Both streptavidin‐fluor conjugates were used to stain dimethyl sulfoxide (DMSO) control samples to further verify the absence of significant frequencies of nonspecific streptavidin‐binding B cells.

Newell K.L., Waldran M.J., Thomas S.J., Endy T.P, & Waickman A.T. (2022). Simultaneous analysis of antigen‐specific B and T cells after SARS‐CoV‐2 infection and vaccination. Cytometry, 101(6), 474-482.

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Multiparameter Flow Cytometry Analysis

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Apoptosis was measured by staining with annexin V FITC (BD Biosciences).
To assay Igλ usage, splenocytes were depleted of red blood cells and
stained with anti-CD21-FITC, anti-CD23-PE, anti-B220-PerCP and
anti-Igλ_1,2,3-biotin (BD Biosciences or Tonbo
Biosciences). The biotinylated antibody was detected with streptavidin-
allophycocyanin (APC) (BD Biosciences). Bone marrow cells were depleted of red
blood cells and stained with anti-B220 FITC,
anti-Igλ_1,2,3-biotin, anti-B220-PerCP, and anti-CD93/AA4.1-APC
(BD Biosciences or Tonbo Biosciences). The biotinylated antibody was detected
with streptavidin-PE (BD Biosciences). T3 cells were identified by staining
splenocytes with anti-B220 FITC, anti-CD23-PE, anti IgM-PerCP, and anti-AA4.1
APC (BD Biosciences or Tonbo Biosciences). In studies with the MD4 anti-HEL Ig
transgene system, splenocytes were depleted of red blood cells and stained with
combinations of anti-IgMb-FITC, anti-IgMa-PE, anti-B220 PerCP, anti-CD19 APC
(antibodies from BD Biosciences or Tonbo Biosciences), and hen egg lysozyme
(Sigma) labeled with Alexa 488 using an Alexa Fluro 488 Microscale Protein
Labeling Kit (Molecular Probes) according to the manufacturer’s
instructions.
Samples were run on a FACS Calibur (Becton Dickinson). Data were analyzed
with CellQuest (BD Biosciences) or Flowjo (Treestar).

Ottens K., Hinman R.M., Barrios E., Skaug B., Davis L.S., Li Q.Z., Castrillon D.H, & Satterthwaite A.B. (2018). Foxo3 promotes apoptosis of BCR-stimulated immature B cells, thus limiting the window for receptor editing. Journal of immunology (Baltimore, Md. : 1950), 201(3), 940-949.

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Annexin V Apoptosis Assay for A549 and Capan-I Cells

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Wild-type or MET^−/− A549 (5 × 10⁴ cells/well) or Capan-I (10⁵ cells/well) cells were seeded in six-well ultralow attachment surface plates in medium plus 2% FCS (A549) or 20% FCS (Capan-I), with or without HGF (25 ng/mL). After 24 h (A549) or 48 h (Capan-I), cell suspensions were collected, washed in PBS, and resuspended in 100 μL of Annexin V binding buffer (BD Biosciences) containing 2.5 μL of biotin-conjugated Annexin V (BD Biosciences) and incubated for 15 min at room temperature. Following one wash in PBS, cells were incubated with 0.5 μL of streptavidin–allophycocyanin (APC; BD Biosciences) in 100 μL of Annexin V binding buffer (BD Biosciences) for an additional 15 min at room temperature in the dark. Then, 7-AAD dye (BD Biosciences) was added right before the acquisition with FACSDiva v9.2 software on a BD FACSCelesta Cell Analyzer. Post-acquisition analysis was performed using FlowJo v10.8.1 software.

Modica C., Cortese M., Bersani F., Lombardi A.M., Napoli F., Righi L., Taulli R., Basilico C, & Vigna E. (2023). Genetic Ablation of the MET Oncogene Defines a Crucial Role of the HGF/MET Axis in Cell-Autonomous Functions Driving Tumor Dissemination. Cancers, 15(10), 2742.

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