Animal husbandry and experiments were performed in accordance with the Division of Laboratory Animal Medicine, University of Rochester, state and federal law, and National Institutes of Health policies. University of Rochester Institutional Animal Care and Use Committee specifically approved all animal work. Wild type C57BL/6J breeders were purchased from Jackson Laboratories and bred in house. Eight-week-old or 18 month-old male C57BL/6J mice were intraperitoneally injected with 100 mg/kg OXA or PBS twice per week. After eight weeks of treatment, whole-body and toxicity measures were conducted. Body weight was measured in grams. Blood samples were collected and serum alanine aminotransferase (ALT) and creatinine were measured using ALT assay kit and Creatinine assay kit (Cayman Chemicals) respectively, per manufacturer instructions. Body fat percentage, lean mass, and bone mineral density (BMD) were measured by Dual Energy X-ray Absorptiometry (DEXA) using LUNAR PIXImus 2 scanner.
Alt assay kit
Manufactured by Cayman Chemical
Sourced in United States, Japan
The ALT assay kit is a laboratory product that measures the activity of the enzyme alanine aminotransferase (ALT) in biological samples. ALT is an enzyme found in various tissues, and its measurement is commonly used as a biomarker to assess liver function. The kit provides the necessary reagents and protocols to perform the ALT activity assay, allowing researchers and clinical laboratories to quantify ALT levels accurately.
Automatically generated - may contain errors
Lab products found in correlation
Cholestech ldx lipid monitoring system, by Thermo Fisher Scientific (2 mentions)
Ultrasensitive insulin elisa kit, by Crystal Chem (2 mentions)
Creatinine assay kit, by Cayman Chemical (1 mentions)
Enzychrom triglyceride assay kit, by BioAssay Systems (1 mentions)
Enzychrom free fatty acid kit, by BioAssay Systems (1 mentions)
Powerscan ht, by Sumitomo Pharma (1 mentions)
C57bl 6 tg cag egfp, by Japan SLC (1 mentions)
C57bl 6j, by Japan SLC (1 mentions)
4 protocols using alt assay kit
1
Long-term Oxaliplatin Exposure in Mice
2
Comprehensive Metabolic Profiling Protocol
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Blood samples were obtained under the fasted condition, and glucose level was determined using a Precision QID glucometer (MediSense Inc., Bedford, MA, USA). Serum cholesterol was measured using Cholestech LDX Lipid Monitoring System (Fisher Scientific, Pittsburgh, PA, USA). Serum triglycerides were measured using EnzyChrom™ Triglyceride Assay Kit (BioAssay systems, Hayward, CA, USA). Serum FFAs were determined using the EnzyChrom™ free fatty acid kit (BioAssay systems). Serum fasting insulin was assayed using the Ultra Sensitive Insulin ELISA Kit (Crystal Chem, Inc., Downers Grove, IL, USA). Fasting whole-body insulin sensitivity was estimated with the HOMA-IR according to the formula [fasting plasma glucose (mg/dL) × fasting plasma insulin (mU/mL)]/405. ALT was assayed using an ALT Assay Kit (Cayman Chemical, Ann Arbor, MI, USA).
3
Aging Study on C57BL/6 Mice
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C57BL/6J and C57BL/6-Tg (CAG-EGFP) female mice were purchased from Japan SLC (Shizuoka, Japan) and CLEA Japan (Tokyo, Japan). Young mice were 2–3 months-old, whereas those older than 24 months were regarded as aged. Mice with grossly visible tumors, hepatomegaly or splenomegaly were excluded from the study. Some but not all mice were assessed for body weight, liver weight, lung weight and serum ALT levels before the experiments began. For the quantification of serum ALT levels, blood was sampled from the heart and centrifuged to separate serum for ALT measurements using an ALT assay kit (#700,260, Cayman Chemical, Ann Arbor, Michigan) and a microplate reader (PowerScanHT; DS Pharma Biomedical, Osaka, Japan). All experiments were carried out following the guidelines of Osaka University Committee for animal and recombinant DNA experiments. Mice were handled and maintained according to the Osaka University guidelines for animal experimentation.
4
Comprehensive Metabolic Profiling Protocol
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Blood samples were obtained under the fasted condition and glucose level was determined using a Precision QID glucometer (MediSense Inc., Bedford, MA). Serum cholesterol and triglycerides were assayed using Cholestech LDX Lipid monitoring System (Fisher Scientific, Pittsburgh PA). Serum fatty acids were determined using the EnzyChrom™ FFA kit (BioAssay systems, Hayward, CA). Serum fasting insulin was assayed using the Ultra Sensitive Insulin ELISA Kit (Crystal Chem, Inc., Downers Grove, IL, USA). Fasting whole-body insulin sensitivity was estimated with the homeostasis model assessment of insulin resistance (HOMA-IR) according to the formula [fasting plasma glucose (mg/dL) x fasting plasma insulin (μU/mL)]/405. Serum alanine transaminase (ALT) was assayed using an ALT assay kit (Cayman Chemical, Ann Arbor MI).
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