Log In Sign up
The largest database of trusted experimental protocols

Adp assay kit

Manufactured by Abcam
Visit Supplier
Sourced in United Kingdom

The ADP Assay Kit is a fluorometric assay designed to quantify the amount of ADP (Adenosine Diphosphate) in biological samples. The kit uses a proprietary enzyme and substrate to produce a fluorescent signal proportional to the ADP concentration in the sample.

Automatically generated - may contain errors

Lab products found in correlation

Atp assay kit, by Abcam (3 mentions) L lactate assay kit, by Abcam (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Pyruvate assay kit, by Abcam (1 mentions) Hydrogen peroxide assay kit, by Abcam (1 mentions) Enspire multimode, by PerkinElmer (1 mentions) Quantikine elisa mouse il 1β, by R&D Systems (1 mentions) Potassium edta microtubes, by Sarstedt (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) Epoch microplate reader, by Agilent Technologies (1 mentions) Human thrombospondin 1 quantikine elisa kit, by R&D Systems (1 mentions) Varioskan flash microplate reader, by Thermo Fisher Scientific (1 mentions) Versamax microplate reader, by Molecular Devices (1 mentions) Cgmp assay kit, by R&D Systems (1 mentions)

10 protocols using adp assay kit

1

Measuring ADP Levels in Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
ADP levels in whole-cell lysates of primary neurons and slices were measured using an ADP Assay Kit (cat: ab83359, Abcam, United Kingdom), according to the manufacturer’s instructions (Chen et al., 2000 (link)).
Cisternas P., Oliva C.A., Torres V.I., Barrera D.P, & Inestrosa N.C. (2019). Presymptomatic Treatment With Andrographolide Improves Brain Metabolic Markers and Cognitive Behavior in a Model of Early-Onset Alzheimer’s Disease. Frontiers in Cellular Neuroscience, 13, 295.
+ Open protocol
+ Expand
2

Quantitative Metabolite Profiling in MEFs

Check if the same lab product or an alternative is used in the 5 most similar protocols
For metabolites measurements, L-Lactate Assay Kit (ab65330), Pyruvate Assay Kit (ab65342), ADP assay kit (ab83359), ATP Assay Kit (ab83355), and Hydrogen Peroxide Assay Kit (ab102500) were purchased from Abcam and performed according to manufacturer’s protocols for the assays. In short, NM1 WT, KO, and KO + NM1 MEFs were grown overnight in 6 well plates (300,000 cells/well), after which they were trypsinized, washed in PBS, and resuspended in the appropriate assay buffer. The protein concentration of each sample was quantified using the Pierce BCA protein assay kit (ThermoFisher Scientific). The amount of each metabolite was measured fluorometrically according to the manufacturer’s recommendations and normalized to the total protein concentration of each sample. Each experiment was repeated at least 3 times with 6 replicates per sample per assay and final graphs are compiled from all experiments together.
Venit T., Sapkota O., Abdrabou W.S., Loganathan P., Pasricha R., Mahmood S.R., El Said N.H., Sherif S., Thomas S., Abdelrazig S., Amin S., Bedognetti D., Idaghdour Y., Magzoub M, & Percipalle P. (2023). Positive regulation of oxidative phosphorylation by nuclear myosin 1 protects cells from metabolic reprogramming and tumorigenesis in mice. Nature Communications, 14, 6328.
+ Open protocol
+ Expand
3

Quantifying Cellular ADP Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded at 7.5 × 105 cells per well in a 6-well plate and grown overnight (12 h). After removing the media and washing cells, PBS (250 µL) was added, and cells were incubated for 15 min. PBS was then collected in ice-cold microcentrifuge tubes and spun at 300× g for 2 min at 4 °C. Supernatants were transferred to a black 96-well plate. ADP was included to ensure the selectivity of the assay. ADP was measured using an ADP assay kit (Abcam) according to the manufacturer’s instructions. Fluorescence was measured at Ex/Em 535/587 nm using a plate reader (EnSpire Multimode, PerkinElmer®, Waltham, MA, USA).
Elaskalani O., Domenchini A., Abdol Razak N.B., E. Dye D., Falasca M, & Metharom P. (2020). Antiplatelet Drug Ticagrelor Enhances Chemotherapeutic Efficacy by Targeting the Novel P2Y12-AKT Pathway in Pancreatic Cancer Cells. Cancers, 12(1), 250.
+ Open protocol
+ Expand
4

Measuring ADP Levels in Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following drug treatment, ADP levels were measured in whole-cell lysates from primary neurons and slices using an ADP Assay Kit (Cat: ab83359, Abcam, UK), according to the manufacturer’s instructions [45 (link)].
Cisternas P., Martinez M., Ahima R.S., Wong G.W, & Inestrosa N.C. (2018). Modulation of Glucose Metabolism in Hippocampal Neurons by Adiponectin and Resistin. Molecular neurobiology, 56(4), 3024-3037.
+ Open protocol
+ Expand
5

Quantifying Plasma and Bone Marrow ADP, IL-1β, and TNNI3

Check if the same lab product or an alternative is used in the 5 most similar protocols
Venous blood was acquired by tail vein puncture in potassium-EDTA microtubes (Sarstedt) and spun down for 8 min at 3.000 G to retrieve plasma. 50 µl plasma was used to measure levels of ADP and IL-1β, using ADP assay kit (Abcam) and Quantikine ELISA Mouse IL-1β (R&D) according to the manufacturers’ protocols. For bone marrow (BM), femoral bones were flushed in 5 mM EDTA (Sigma-Aldrich), spun down for 5 min at 4.000 G and supernatant was used for ADP ELISA (Abcam). For TNNI3-ELISA, blood was acquired by tail vein puncture in potassium-EDTA microtubes (Sarstedt) and spun down for 15 min at 1.000 G to retrieve plasma. 100 µl of 1:10 diluted plasma was used to measure protein levels of TNNI3, using Mouse TNNI3/Cardiac Troponin I kit (Life Span Biosciences) following the manufacturer’s protocol.
Seung H., Wrobel J., Wadle C., Bühler T., Chiang D., Rettkowski J., Cabezas-Wallscheid N., Hechler B., Stachon P., Maier A., Weber C., Wolf D., Duerschmied D., Idzko M., Bode C., von zur Mühlen C., Hilgendorf I, & Heidt T. (2022). P2Y12-dependent activation of hematopoietic stem and progenitor cells promotes emergency hematopoiesis after myocardial infarction. Basic Research in Cardiology, 117(1), 16.
+ Open protocol
+ Expand
6

Metabolic Profiling of Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lactate production of CAL-62 and BHT-101 cells was measured using the L-lactate Assay kit (ab65331, abcam) as the protocol. ADP levels was determined using ADP assay kit (ab83359, abcam). ATP levels were determined using ATP assay kit (ab83355, abcam). Protein concentration was detected for normalization. A450 was measured using Epoch microplate reader (BioTek).
Liu K., Du Y., Li H, & Lin X. (2022). Identification of super-enhancer-associated transcription factors regulating glucose metabolism in poorly differentiated thyroid carcinoma. Genetics and Molecular Biology, 45(3), e20210370.
+ Open protocol
+ Expand
7

Evaluation of Platelet Activation Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
ADP plasma levels were evaluated by an ADP Assay Kit (Abcam). sP-Selectin (soluble P-selectin) was determined utilizing a Human sP-selectin Platinum ELISA (Thermo Scientific). For the quantification of thrombospondin (TSP-1) a Human Thrombospondin-1 Quantikine ELISA Kit (R&D Systems) was used. Soluble CD40L concentration was determined using a Human sCD40L (soluble CD40 ligand) Platinum ELISA Kit (Thermo Scientific). All assays were performed according to the manufacturers' protocol. All plates were measured by a VersaMax microplate reader (Molecular Devices). ADP Assay Kit plates were read on a Varioskan Flash microplate reader (Thermo Scientific).
Scherz T., Hofbauer T.M., Ondracek A.S., Simon D., Sterz F., Testori C., Lang I.M, & Mangold A. (2021). Mild Therapeutic Hypothermia Alters Hemostasis in ST Elevation Myocardial Infarction Patients. Frontiers in Cardiovascular Medicine, 8, 707367.
+ Open protocol
+ Expand
8

Quantitative ATP and ADP Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The ATP or ADP in the liver extract was determined using a ATP Assay Kit (Abcam) or ADP Assay Kit (Abcam), respectively, according to the manufacturer’s instructions. Data were normalized by the amount of protein present in the supernatant.
Zhang C., Seo J., Murakami K., Salem E.S., Bernhard E., Borra V.J., Choi K., Yuan C.L., Chan C.C., Chen X., Huang T., Weirauch M.T., Divanovic S., Qi N.R., Thomas H.E., Mercer C.A., Siomi H, & Nakamura T. (2018). Hepatic Ago2-mediated RNA silencing controls energy metabolism linked to AMPK activation and obesity-associated pathophysiology. Nature Communications, 9, 3658.
+ Open protocol
+ Expand
9

Platelet-Mediated Nitric Oxide and TXB2 Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hu-platelets (108) were incubated with 5 μg rLep-vWA-I or rLep-vWA-II in MTB at 37 °C for 1 h. The Hu-platelets were lysed and then centrifuged as above. The OD540 or OD450 values reflecting the nitric oxide (NO) or cGMP levels in the supernatants were measured using a Griess's Diazotization NO Assay Kit (Promega, USA) or a cGMP Assay Kit (R&D) by spectrophotometry. The supernatants were diluted at 1:50 with the assay buffer and then the OD450 values reflecting TXB2 levels were measured using a TXB2 Assay Kit (R&D) by spectrophotometry. Besides, the Hu-platelets were precipitated as above for homogenization in the assay buffer and then centrifuged at 10,000 ×g for 10 min. The supernatants were diluted with 50-fold volumes of the assay buffer for detecting the released ADP at the OD570 using an ADP Assay Kit (Abcam) by spectrophotometry. In the detection, 10 μg rHu-vWF plus 150 μM ristocetin (rHu-vWF/ristocetin) were used as the control [26 (link),28 (link)].
Fang J.Q., Imran M., Hu W.L., Ojcius D.M., Li Y., Ge Y.M., Li K.X., Lin X, & Yan J. (2018). vWA proteins of Leptospira interrogans induce hemorrhage in leptospirosis by competitive inhibition of vWF/GPIb-mediated platelet aggregation. EBioMedicine, 37, 428-441.
+ Open protocol
+ Expand
10

Quantifying ADP Levels in Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
ADP levels in whole-cell lysates of primary neurons and slices were measured using an ADP Assay Kit (cat: ab83359, Abcam, UK), according to the manufacturer’s instructions (Chen et al. 2000 ).
Cisternas P., Zolezzi J.M., Martinez M., Torres V.I., Wong G.W, & Inestrosa N.C. (2018). Wnt-induced activation of glucose metabolism mediates the in vivo neuroprotective roles of Wnt signaling in Alzheimer disease. Journal of neurochemistry, 149(1), 54-72.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!