Eo771

Both murine tumor cell lines used were of C57BL/6 origin (H2^b). The mammary adenocarcinoma cell line EO771 was purchased from TEBU-Bio (Offenbach, Germany) and RMA-S cells (a Rauscher virus induced T lymphoma) were kindly provided by Günter Hämmerling, DKFZ Heidelberg, Germany. RMA-S cells were propagated in complete RPMI medium containing RPMI 1640 supplemented with Glutamax (Life technologies / Thermo Fisher), 10% FCS (Biochrom, Berlin, Germany), and 1% penicillin-streptomycin (Life technologies / Thermo Fisher). EO771 cells were cultured in complete RPMI medium containing 1 mM HEPES buffer (Sigma-Aldrich, Saint Louis, MO).

MCF10A, MDA-MB-231, MDA-MB-468, Hs578T, and three mouse breast cancer cell lines 4T1, EMT6, EO771 were purchased from the American Type Culture Collection (ATCC). 293A, MDA-MB-231, MDA-MB-468, and EO771 cells were cultured in DMEM supplemented with 10% FCS (Sigma-Aldrich). Hs578T cells were grown in DMEM with 10% FCS and 0.01 mg/ml human insulin (Sigma-Aldrich). MCF10A normal breast epithelial cells were grown in DMEM/F12 medium supplemented with 20 ng/ml epithelial growth factor (EGF; Thermo Fisher Scientific), 100 ng/ml cholera toxin (Sigma-Aldrich), 10 μg/ml insulin (Sigma-Aldrich), 0.5 mg/ml hydrocortisone (Sigma-Aldrich), and 5% horse serum (Thermo Fisher Scientific). 4T1 cells were cultured in Roswell Park Memorial Institute 1640 medium with 10% FBS. EMT6 cells were grown in Waymouth MB 752/1 Medium with 2 mM L-glutamine and 15% FBS. All the cell lines were tested to verify that they were free of mycoplasma contamination.