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2 protocols using sodium fluoroacetate

1

Sodium Fluoroacetate Toxicity Assay

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Sodium fluoroacetate (CAT# N-13216–250MG) was purchased from Chem Service Inc. (West Chester, PA, USA) and dissolved in sterile water for injection (CAT# A1287301) (Thermo Fisher Scientific, Waltham, MA, USA). Formalin (CAT# 5705), hematoxylin (CAT# 72404), and eosin (CAT# 7111) were obtained from Thermo Fisher Scientific. 5% 5-sulfosalicylic acid (SSA) (CAT# S7422), glutathione (CAT# CS0260), 5,5’-dithiobis(2-nitrobenzoic acid) (DTNB) (CAT# D8130), dimethyl sulfoxide (DMSO) (CAT# D2650), glutathione reductase (CAT# G3664), glutathione assay buffer (CAT# CS02060), and β-nicotinamide adenine dinucleotide 2’-phosphate reduced tetrasodium salt hydrate (NADPH) (CAT# NADPH-RO) were obtained from MilliporeSigma (St. Louis, MO, USA).
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2

Pharmacological Agents Evaluation Protocol

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LPS, minocycline, paeonol, MRS2179 ammonium salt hydrate, MPEP hydrochloride, bicuculline methiodide, DL-2-amino-5-phosphonovaleric acid (APV), and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) were purchased from the Sigma Chemical Company (St Louis, MO, USA), sodium fluoroacetate was purchased from Chem Service, Inc. (West Chester, USA), (N)-methanocarba-2MeSADP (MRS2365) was purchased from R&D Systems, Inc. (Minneapolis, USA), and tetrodotoxin (TTX) was purchased from Alomone Labs Ltd. (Jerusalem, Israel). paeonol was prepared in ethanol; MPEP and CNQX were prepared in DMSO; distilled water was used for all other drug stocks.
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