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α drp1

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α-Drp1 is a primary antibody that recognizes the Drp1 protein. Drp1 is a dynamin-related protein that plays a crucial role in mitochondrial fission.

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Lab products found in correlation

α tubulin, by Cell Signaling Technology (2 mentions) α gapdh, by Cell Signaling Technology (2 mentions) α actin, by Cell Signaling Technology (2 mentions) α erk1 2, by Abcam (2 mentions) α pt202 y204erk 1 2, by Cell Signaling Technology (2 mentions) α hk2, by Cell Signaling Technology (2 mentions) α hif1α, by Cell Signaling Technology (2 mentions) α actin, by Abcam (1 mentions) Ab184247, by Abcam (1 mentions) Ab110328, by Abcam (1 mentions) Ab2799, by Abcam (1 mentions)

3 protocols using α drp1

1

Western Blot Analysis of Cellular Proteins

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Whole cell lysates were prepared in RIPA buffer and equivalent protein amounts (generally 50 μg) were resolved by SDS-Page. Gels were transferred to PVDF membranes and immunoblotted with the indicated antibodies. List of antibodies used in this study: α-Drp1 (Abcam), α-Erk1/2, α-pT202/Y204Erk 1/2, α-HK2, α-HIF1α, α-Tubulin, α-Actin, α-GAPDH (Cell Signaling Technologies).
Nagdas S., Kashatus J.A., Nascimento A., Hussain S.S., Trainor R.E., Pollock S.R., Adair S.J., Michaels A.D., Sesaki H., Stelow E.B., Bauer T.W, & Kashatus D.F. (2019). Drp1 Promotes KRas-Driven Metabolic Changes to Drive Pancreatic Tumor Growth. Cell reports, 28(7), 1845-1859.e5.
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2

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell lysates were prepared in RIPA buffer and equivalent protein amounts (generally 50 μg) were resolved by SDS-Page. Gels were transferred to PVDF membranes and immunoblotted with the indicated antibodies. List of antibodies used in this study: α-Drp1 (Abcam), α-Erk1/2, α-pT202/Y204Erk 1/2, α-HK2, α-HIF1α, α-Tubulin, α-Actin, α-GAPDH (Cell Signaling Technologies).
Nagdas S., Kashatus J.A., Nascimento A., Hussain S.S., Trainor R.E., Pollock S.R., Adair S.J., Michaels A.D., Sesaki H., Stelow E.B., Bauer T.W, & Kashatus D.F. (2019). Drp1 Promotes KRas-Driven Metabolic Changes to Drive Pancreatic Tumor Growth. Cell reports, 28(7), 1845-1859.e5.
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3

Immunoblotting and Immunocytochemistry Protocols

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α‐actin (Abcam, Cell Signaling Technology, 3700S, 1:1000 for WB), α‐Drp1 (Abcam, ab184247, 1:250 for ICC, 1:1000 for WB), α‐phospho Drp1(S616) (Cell Signaling Technology, 3455S, 1:250 for ICC, 1:1000 for WB), α‐FXN (Abcam, ab110328, 1:1000 for WB), α‐GRP75 (Abcam, ab2799, 1:250 for ICC).
Johnson J., Mercado‐Ayón E., Clark E., Lynch D, & Lin H. (2021). Drp1‐dependent peptide reverse mitochondrial fragmentation, a homeostatic response in Friedreich ataxia. Pharmacology Research & Perspectives, 9(3), e00755.
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