Fumonisin b2

Manufactured by Merck Group

Sourced in Belgium, United States

Fumonisin B2 is a laboratory standard used for the identification and quantification of this mycotoxin in various samples. It serves as a reference material for analytical methods and quality control purposes.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Fumonisin (4 citations) Fumonisin B1 and B2 (2 citations)

4 protocols using fumonisin b2

Quantitative Determination of Mycotoxins

Check if the same lab product or an alternative is used in the 5 most similar protocols

LC–MS grade methanol, formic acid and QuEChERS [41 (link)] kit were purchased from Sigma Aldrich. Deionised water, water purified through Milli-Q system (Millipore) was used to prepare the standard solutions and for LC/MS analysis. The following mycotoxin standards were used: aflatoxins mix of AFG1, AFB1, AFG2, AFB2 (10 mg / L), ochratoxin A (OTA, 10 mg / L), ochratoxin B (OTB, 10 mg / L) fumonisin B1 (FB1, 50 mg / L), fumonisin B2 (FB2, 50 mg / L) were procured from Sigma Aldrich.

Al Jabir M., Barcaru A., Latiff A., Jaganjac M., Ramadan G, & Horvatovich P. (2019). Dietary exposure of the Qatari population to food mycotoxins and reflections on the regulation limits. Toxicology Reports, 6, 975-982.

+ Open protocol

+ Expand

Quantitative Analysis of Mycotoxins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reference substances of aflatoxin B₁ (AFB₁), aflatoxin B₂ (AFB₂), aflatoxin G₁ (AFG₁), aflatoxin G₂ (AFG₂), deoxynivalenol (DON), fumonisin B₁ (FB₁), fumonisin B₂ (FB₂), T-2 toxin (T-2), HT-2 toxin (HT-2), ochratoxin A (OTA), zearalenone (ZEN), zearalanone (ZAN), α-zearalenol (α-ZEL), β-zearalenol (β-ZEL), and β-zearalanol (β-ZAL) were purchased from Sigma Aldrich Chemicals (St. Louis, MO). Standard stock solutions (100 µg/mL) were diluted to mixed working solutions with 35% methanol and sample matrix solutions. Concentrations of the mixed standard working solutions are shown in Table S3.
Methanol (LC/MS grade) and acetonitrile (LC grade) were purchased from Merck KGaA (Darmstadt, Germany). Formic acid, acetic acid, and ammonium acetate, both of LC/MS grade, were supplied by Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). QuEChERS purifier was obtained from Meizheng Bio-Tech Co., Ltd. (Wuxi, Jiangsu, China), and it consisted of octadecyl silica (C18), silica, graphitized carbon black (GCB), magnesium sulfate (MgSO₄), and primary secondary amine (PSA).

Zhang Y., Kuang F., Liu C., Ma K., Liu T., Zhao M., Lv G, & Huang H. (2023). Contamination and Health Risk Assessment of Multiple Mycotoxins in Edible and Medicinal Plants. Toxins, 15(3), 209.

+ Open protocol

+ Expand

Comprehensive Mycotoxin Standards for Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mycotoxin standards comprising of aflatoxin B₁ (AFB₁), aflatoxin B₂ (AFB₂), aflatoxin G₁ (AFG₁), aflatoxin G₂ (AFG₂), fumonisin B₁ (FB₁), fumonisin B₂ (FB₂), deepoxy-deoxy nivalenol (DOM), 15-acetyl-deoxynivanelol (15-ADON), neosolaniol (NEO), OTA, alternariol (AOH), alternariol monomethyl ether (AME), zearalenone (ZEN), nivalenol (NIV), deoxynivanelol (DON), 3-acetyl-deoxynivanelol (3-ADON), sterigmatocystin (STE), roquefortine C (ROQ C), enniatin B (ENN B), fusarenon-X (FUS-X), HT-2 toxin (HT-2) and zearalanone (ZAN) were obtained from Sigma-Aldrich (Bornem, Belgium). Fumonisin B₃ (FB₃) was procured at Promec Unit (Tynberg, South Africa), while T-2 toxin (T-2) and diacetoxyscirpenol (DAS) were purchased from Biopure Referenzsubstanze (Tulln, Austria).

Adekoya I., Njobeh P., Obadina A., Chilaka C., Okoth S., De Boevre M, & De Saeger S. (2017). Awareness and Prevalence of Mycotoxin Contamination in Selected Nigerian Fermented Foods. Toxins, 9(11), 363.

+ Open protocol

+ Expand

Multiplex Mycotoxin Immunoassay Development

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sodium borohydride (NaBH₄), HAuCl₄·3H₂O, trisodium citrate, maleic anhydride, protein G, bovine serum albumin (BSA), GOx, and glucose, FB₁, fumonisin B₂ (FB₂), fumonisin B₃ (FB₃), citinin (CIT), deoxyivalenol (DON), aflatoxin B₁ (AFB₁) and Trichothecenes 2 toxins (T-2), 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), 3, 3’, 5, 5’-Tetramethylbenzidine liquid system and H₂O₂ were purchased from Sigma-Aldrich Chemical (St. Louis, MO, USA). An H₂O₂ quantitative assay kit (water-compatible) was purchased from Sangon Biotech (Shanghai, China) (www.sangon.com). Anti-FB₁ monoclonal antibody ascitic fluids (anti-FB₁ mAb 6.5 mg/mL) were provided by Prof. Xu research group (Nanchang University, China). 96-well microplates (high binding, white) were obtained from Costar Inc. (Cambridge, MA, USA). Other reagents were of analytical grade were purchased from Sinopharm Chemical Corp (Shanghai, China). Aqueous solutions for the immunoassay experiments were prepared using Millipore water (Elix-3þ Milli-QA, Millipore, France).

Zhan S., Zheng L., Zhou Y., Wu K., Duan H., Huang X, & Xiong Y. (2019). A Gold Growth-Based Plasmonic ELISA for the Sensitive Detection of Fumonisin B1 in Maize. Toxins, 11(6), 323.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!