Sodium chloride (nacl)

Standards of all metabolites were purchased from several vendors, such as Sigma-Aldrich (St. Louis, MO, USA) and Tokyo Chemical Industry (Kita-ku, Tokyo, Japan). HPLC-grade water, methanol (MeOH), and acetonitrile (ACN) were purchased from J.T. Baker Inc. (Phillipsburg, NJ, USA). LC-MS-grade formic acid was purchased from Thermo Scientific (Waltham, MA, USA). Spin-X centrifuge filter 0.45 μm with cellulose acetate was purchased from Costar (Corning, NY, USA). Triethylamine (TEA), hematoxylin and eosin (H&E), PPE, and LPS (Escherichia coli O111:B4) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS) was purchased from Gibco (Grand Island, NY, USA). Toluene, diethyl ether (DEE), ethyl acetate (EA), dichloromethane (DCM), and sodium chloride (pesticide grade) were purchased from Kanto Chemical (Chuo-ku, Tokyo, Japan). Sodium hydroxide (NaOH) and sulfuric acid were obtained from Daejung Reagents Chemicals (Siheung, South Korea). O-methoxyamine hydrochloride (MO) and N-methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA) + 1% tert-butyldimethylchlorosilane (TBDMCS) were obtained from Thermo Scientific (Bellefonte, PA, USA). All other chemicals were of analytical grade.

All oligonucleotides were purchased from Eurofins Genomics (Luxembourg, Luxembourg). The sequences are indicated in the Supplementary Materials Table S1.
Phenazine methosulfate (PMS), 2,6-dichlorophenolindophenol (DCIP), d(+)-glucose, sodium chloride, 2-amino-2-hydroxymethyl-propane-1,3-diol (Tris), glycerol, and Tween-20 were purchased from Kanto Chemical Co. Inc. (Tokyo, Japan). Kanamycin sulfate and d-desthiobiotin were purchased from Sigma-Aldrich Co. LLC (St. Louis, MO, USA). A self-assembled monolayer (SAM) forming thiol reagent (dithiobis(succinimidyl undecanoate)) was purchased from Dojindo Laboratories Co., Ltd. (Kumamoto, Japan). LB broth, the bacterial host strain Escherichia coli BL21(DE3), and the expression vector pET30c(+) were purchased form Merck KGaA (Darmstadt, Germany). Anti-VEGF antibodies (MAB293 and BAF293) were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). Gold and platinum wires were purchased from TANAKA Kikinzoku (Tokyo, Japan). A silver/silver chloride (3M NaCl) reference electrode RE-1B (Ag/AgCl) was purchased from BAS Inc. (Tokyo, Japan).

CMC (molecular weight = 8–10 × 10⁴ as specified by manufacturer) with a 0.70 degree of substitution was obtained from Daicel Co., (Osaka, Japan). EGDE was purchased from Fujifilm Wako Pure Chemical Co., Ltd. (Osaka, Japan). Liquid paraffin (Moresco white P-100, 19.04 mm²/s) was purchased from Moresco Co., (Kobe, Japan). Sodium hydroxide was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Methanol was purchased from Godo Co., Ltd. (Tokyo, Japan). The SPA was supplied by S. T. Co., (Tokyo, Japan). Trichoderma viride cellulase ONOZUKA R-10 was purchased from Yakult Pharmaceutical Co., Ltd. (Tokyo, Japan). The PBS and sodium acetate buffer used were analytical grade chemicals, i.e., sodium chloride, potassium chloride, sodium dihydrogen phosphate, potassium dihydrogen phosphate, sodium acetate, and acetic acid, purchased from Kanto Chemical Co., Inc. (Tokyo, Japan).

Irgafos 168 and Erucamide were purchased from Tokyo Chemical Industry Co. (Tokyo, Japan). Acetone, methanol, chloroform, sodium chloride, potassium chloride, sodium sulfate, ammonium chloride, urea, lactic acid, sodium hydroxide, and 2-propanol were purchased from Kanto Chemical Co. (Tokyo, Japan). Formic acid was purchased from Fluka (Charlotte, NC, USA). Water was generated by PURELAB frex-3 (ELGA, High Wycombe, UK). These reagents were used without further purification. Stock solutions of Irgafos 168 and Erucamide were prepared by dissolving 0.1 g of reagents in 100 mL of Acetone and methanol, respectively. Working solutions were prepared by diluting the stock solutions with methanol.