Omics explorer v 3.2

Further details and an outline of resources used in this work can be found in the Supplemental Information. Briefly, microarray analysis was performed as previously described (Fang et al., 2017 (link)), using Agilent SurePrint G3 Mouse 8×60K Microarray. Gene network and pathway analysis were carried out using Qlucore Omics Explorer (v.3.2) (Qlucore) and ingenuity pathway analysis (Ingenuity Systems QIAGEN). Animal studies were approved by the Institutional Animal Care and Use committee of Yale University School of Medicine. WT C57BL/6 mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). Generation of miR-33^−/− mice was accomplished with the assistance of Cyagen Biosciences. Induction of diet-induced obesity was accomplished by feeding a HFD (60% fat) (D12492; Research Diets, New Brunswick, NJ, USA). Measurements of metabolic function, circulating lipids, and adipocyte proliferation/function were carried out using established techniques. Statistical differences were measured using an unpaired two-sided Student’s t test or one-way ANOVA with Bonferroni correction for multiple comparisons. Normality was checked using the Kolmogorov-Smirnov test. A nonparametric test (Mann-Whitney) was used when data did not pass the normality test. p < 0.05 was considered statistically significant.