Fotemustine

Manufactured by Merck Group

Sourced in Germany

Fotemustine is a laboratory chemical used in research applications. It is a cytotoxic agent with antineoplastic properties. The core function of Fotemustine is to inhibit cell division and tumor growth.

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2 protocols using fotemustine

Cytotoxicity Assay for Cancer Drug Treatments

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Drug treatments were performed for at least 48 h in six-well plates (2.5 × 10⁵ cells seeded) for RNA/protein extraction or 96-well plates (2500 cells per well seeded) for viability assays. Stock solutions of etoposide (10 mM, dimethylsulfoxide), cisplatin (1670 mM, sodium chloride, 0.9%), temozolomide (10 mM, dimethylsulfoxide) and nutlin-3a (10 mM, dimethylsulfoxide), (all from Sigma-Aldrich, Munich, Germany) and fotemustine (50 mg/ml, ethanol, 80%) were used. fotemustine was received either directly from the hospital and administered to cells at day of preparation, or received from company (Sigma-Aldrich). Viability was determined by MTT assay using 50 μl of MTT solution (5 mg/ml) added 48 h after drug treatment. Concentration of dimethylsulfoxide-solubilized formazan was detected at 570 nm after 1 h of incubation.

Redmer T., Walz I., Klinger B., Khouja S., Welte Y., Schäfer R, & Regenbrecht C. (2017). The role of the cancer stem cell marker CD271 in DNA damage response and drug resistance of melanoma cells. Oncogenesis, 6(1), e291-.

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Comprehensive Protein Profiling for Cancer Research

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Antibodies against IKKβ, catalase, SOD1, p21, p27, p-Chk1(S345), Chk1, p-Chk2(T68), Chk2, p-H2AX(S139), H2AX, p-ATM(S1981), ATM, MGMT, PARP, Caspase-3, p-p53(S15), p53, survivin, XIAP, cIAP-1, cIAP-2, Mre11, Rad50, p95/NBS1, were purchased from Cell Signaling (Beverly, MA). Antibodies against Bak, Bcl-2, Bcl-xL, GAPDH, and actinin were purchased from Santa Cruz Biotechnology, Inc. Fotemustine and antibody against Flag (M2) was purchased from Sigma. 8-OHdG ELISA kit, mirin and SC-514 for in vivo experiment were purchased from Abcam. SC-514 for in vitro experiment, carmustine and lomustine were purchased from Enzo Life Sciences. PLX4032, BMS-345541, TPCA-1, LY-2409881, AZD3264, IKK-16 and PS-1145 were purchased form Selleckchem ML 120B dihydrochloride and PF184 were purchased from Tocris Bioscience. Crystal violet, propidium iodide (PI), N-acetyl-L-cysteine (NAC), temozolomide, 3-Amino-1,2,4-triazole (ATZ), hydrogen peroxide solution and 3-(4,5-Dimethyl-2-thiazolyl)−2,5-diphenyl-2H-tetrazolium bromide (MTT) were purchased from Sigma. 6-Carboxy-2′,7′-dichlorofluorescein diacetate (DCF) was purchased from Thermo Scientific.

Tse A.K., Chen Y.J., Fu X.Q., Su T., Li T., Guo H., Zhu P.L., Kwan H.Y., Cheng B.C., Cao H.H., Lee S.K., Fong W.F, & Yu Z.L. (2017). Sensitization of melanoma cells to alkylating agent-induced DNA damage and cell death via orchestrating oxidative stress and IKKβ inhibition. Redox Biology, 11, 562-576.

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