Fastscan d cantilever

Manufactured by Bruker

Sourced in United States

FastScan D cantilevers are high-speed atomic force microscope (AFM) probes designed for rapid imaging and characterization of sample surfaces. They feature a small effective spring constant and low effective mass, enabling high-speed scanning capabilities while maintaining high resolution.

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Lab products found in correlation

Multimode 8 microscope, by Bruker (2 mentions) Dimension fastscan microscope, by Bruker (2 mentions) Peakforce hirs f b cantilevers, by Bruker (2 mentions) Fastscan bio afm system, by Bruker (2 mentions) 1d image analysis software, by Kodak (1 mentions) Dimension fastscan bio afm, by Bruker (1 mentions) Nanoscope analysis software, by Bruker (1 mentions) Dimension fastscan system, by Bruker (1 mentions)

Close spelling variants of this product

FastScan (15 citations) FastScan D (9 citations) Cantilevers (4 citations)

7 protocols using fastscan d cantilever

Visualizing DNA Scaffolds with AFM

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DNA was visualised by PAGE on polyacrylamide gels (15%, 19:1 acyrlamide/bis-acrylamide). Samples were loaded with Blue/Orange loading dye, run at 80 V for 360 minutes and stained with SYBR Gold Nucleic Acid Gel Stain. Imaging was carried out using Kodak 1D image analysis software.
Densitometry was undertaken using ImageJ. The total intensity in each lane was obtained and the relative intensity of each band as a percentage of the total lane intensity was calculated.
For analysing DNA scaffolds containing 0 and 20 base mismatches using AFM, 50 μl of DNA (8–10 ng in total) in 10 mM Tris-EDTA pH 8 buffer was allowed to adsorb on a freshly cleaved mica surface, pre-treated with 10 mM NiCl₂ for 20 minutes in a high humidity chamber. A further 100 μl of 10 mM Tris-EDTA pH 8 buffer was afterwards added. The resulting sample was imaged with a Dimension FastScan Bio AFM (Bruker, USA) in peak force tapping mode, in liquid, with a Fastscan-D cantilever (Bruker, USA) at an amplitude set point of 250 mV and 57.68 mV drive amplitude. All the captured AFM images were analysed with the Nanoscope Analysis software from Bruker.

Corbett S.L., Sharma R., Davies A.G, & Wälti C. (2017). Enhancement of RecA-mediated self-assembly in DNA nanostructures through basepair mismatches and single-strand nicks. Scientific Reports, 7, 41081.

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Atomic Force Microscopy of DNA Origami

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AFM characterization was performed
on freshly cut mica substrates or glass coverslips (prepared as described
above). Four μL of a DOC sample was deposited onto the substrate
for 5 min and then 100 μL of deposition buffer was added to
form a droplet on top of the sample. AFM imaging was performed with
a Dimension-FastScan system from Bruker set to amplitude modulation
mode. Imaging was carried out in liquid with a set-point ratio between
the free amplitude and imaging amplitude of ∼0.7. The FastScan
D cantilever was supplied by Bruker, with a nominal spring constant
of 0.25 N/m. Subnanometer amplitude was used to image DNA docking
strand positions on every DNA origami construct following the method
of ref (56 (link)). Rows of
the images were aligned and flattened using Gwyddion software package.^{57 (link)}

Cervantes-Salguero K., Kadrmas M., Ward B.M., Lysne D., Wolf A., Piantanida L., Pascual G, & Knowlton W.B. (2024). Minimizing Structural Heterogeneity in DNA Self-Assembled Dye Templating via DNA Origami-Tuned Conformations. Langmuir, 40(19), 10195-10207.

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Real-Time Imaging of Chaperone-Mediated Protein Disaggregation

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All AFM imaging was performed in liquid using Peakforce Tapping with either a Dimension FastScan microscope (images presented in Figs 1A and B, and 2A and D, and EV1) or a Multimode 8 (images presented in Fig 1C). FastScan D cantilevers (Bruker) were used with the Dimension FastScan microscope and PeakForce HIRS‐F‐B cantilevers (Bruker) were used with the Multimode 8 microscope. FastScan D cantilevers were operated using a drive frequency of 8 kHz and PeakForce HIRS‐F‐B cantilevers were operated at 4 kHz drive frequency. The areas imaged were 0.8 × 0.25–4 × 2 µm² in size and recorded at 3.5 Hz (FastScan) or 1.75 Hz (Multimode 8) line rate. Images were 512 × 256, 512 × 172 or 384 × 172 pixels in size.
During AFM movie acquisition, disaggregation was initiated by retracting the cantilever tip ~100 nm from the surface and injecting 0.75–1 µM Hsc70, 0.37 – 0.5 µM DNAJB1 and 0.07–0.1 µM Apg2 (always at a Hsc70:DNAJB1:Apg2 molar ratio of 1:0.5:0.1) in disaggregation buffer (HKMD buffer for ‐ATP controls) into the sample droplet. AFM imaging was continued within 1 min of injection of chaperones. The same area of αSyn fibres was imaged for >2 h at either at room temperature or at 30°C.

Beton J.G., Monistrol J., Wentink A., Johnston E.C., Roberts A.J., Bukau B.G., Hoogenboom B.W, & Saibil H.R. (2022). Cooperative amyloid fibre binding and disassembly by the Hsp70 disaggregase. The EMBO Journal, 41(16), e110410.

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High-Resolution Imaging of DNA Nanostructures

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Freshly cleaved mica was treated with 20 mM NiCl₂ before sample deposition. After the folding process was completed, the structures were imaged with a Bruker Bio-FastScan AFM (in Tapping mode) using FastScan-D cantilevers with a force constant of 0.25 N/m (Bruker, Billerica, Massachusetts, USA). This mode has been used to acquire high-resolution images of the studied objects (DNA). The images were typically captured in the retrace direction with a scan rate of 3.95 Hz and a resolution of 1024 samples/line (for ∼660 nm ²), yielding a ∼0.65 × 0.65 nm pixel size. Imaging was done in TAEx1 buffer with 12.5 mM MgCl₂ at room temperature. The buffers and salts were filtered using syringe filters just before use.

Roth Weizman E., Glick Azaria A, & Garini Y. (2022). Conformation of ring single-stranded DNA measured by DNA origami structures. Biophysical Journal, 121(11), 2127-2134.

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Atomic Force Microscopy Imaging Protocols

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All AFM measurements were performed in liquid in PeakForce Tapping imaging on a FastScan Bio AFM system using FastScan D cantilevers (Bruker). Imaging was carried out with a PeakForce Tapping amplitude of 10 nm, at a PeakForce frequency of 8 kHz, at PeakForce setpoints of 5–20 mV, (peak forces of < 100 pN). Images were recorded at 512 × 512 pixels to ensure resolution ≥ 1 nm/pixel at line rates of 3.5 Hz.

Beton J.G., Moorehead R., Helfmann L., Gray R., Hoogenboom B.W., Joseph A.P., Topf M, & Pyne A.L. (2021). TopoStats – A program for automated tracing of biomolecules from AFM images. Methods (San Diego, Calif.), 193, 68-79.

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High-Resolution Atomic Force Microscopy

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All AFM measurements were performed in liquid in PeakForce Tapping imaging on a FastScan Bio AFM system using FastScan D cantilevers (Bruker). Imaging was carried out with a PeakForce Tapping amplitude of 10 nm, at a PeakForce frequency of 8 kHz, at PeakForce setpoints of 5-20 mV, (peak forces of <100 pN). Images were recorded at 512 × 512 pixels to ensure resolution ≥ 1 nm/pixel at line rates of 3.5 Hz.

Beton J.G., Moorehead R., Helfmann L., Gray R., Hoogenboom B.W., Joseph A.P., Topf M., & Pyne A.L.B. (2020). TopoStats – a program for automated tracing of biomolecules from AFM images.

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Disaggregation of α-Synuclein Fibrils by Chaperones

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All AFM imaging was performed in liquid using Peakforce Tapping with either a Dimension FastScan microscope (images presented in Figures 1A,B, Ci and Figures 2 and3) or a Multimode 8 (images presented in Figure 1Cii). FastScan D cantilevers (Bruker) were used with the Dimension FastScan microscope and PeakForce HIRS-F-B cantilevers (Bruker) were used with the Multimode 8 microscope. FastScan D cantilevers were operated using a drive frequency of 8 kHz and PeakForce HIRS-F-B cantilevers were operated at 4 kHz drive frequency. The areas imaged were 0.8 x 0.25 -4 x 2 µm 2 in size and recorded at 3.5 Hz (FastScan) or 1.75 Hz (Multimode 8) line rate.
Images were 512 x 256, 512 x 172 or 384 x 172 pixels in size.
During AFM movie acquisition, disaggregation was initiated by retracting the cantilever tip ~100 nm from the surface and injecting 0.75 -1 µM Hsc70, 0.37 -0.5 µM DNAJB1 and 0.07 -0.1 µM Apg2 (always at a Hsc70:DNAJB1:Apg2 molar ratio of 1:0.5:0.1) in disaggregation buffer (HKMD buffer for -ATP controls) into the sample droplet. AFM imaging was continued within 1 minute of injection of chaperones. The same area of αSyn fibres was imaged for >2 hours at either at room temperature or at 30 °C.

Beton J.G., Monistrol J., Wentink A.S., Johnston E.F., Roberts A., Bukau B., Hoogenboom B.W., & Saibil H. (2021). Cooperative Amyloid Fibre Binding and Disassembly by the Hsp70 disaggregase.

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