Antigen retrieval solution

Manufactured by BioGenex

Sourced in United States

Antigen retrieval solution is a laboratory reagent used to enhance the detection of specific proteins or antigens in tissue samples during immunohistochemical analysis. It is designed to unmask or expose the target antigens that may be hidden or altered due to the fixation process, allowing for improved antibody binding and subsequent visualization of the target protein.

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Lab products found in correlation

H 5000, by Vector Laboratories (1 mentions) Ez dewax, by BioGenex (1 mentions) Mayer s hematoxylin, by Agilent Technologies (1 mentions) Immpact novared peroxidase substrate, by Vector Laboratories (1 mentions) Goat anti nrp1, by R&D Systems (1 mentions) Goat anti nrp2, by R&D Systems (1 mentions)

Close spelling variants of this product

Antigen Retrieval Citra Solution (18 citations) Antigen Retrieval Citra Plus Solution (5 citations) Antigen Retrieval Citra Solution (pH 6) (2 citations) Antigen Retrieval AR-10 Solution (2 citations)

4 protocols using antigen retrieval solution

Immunoblotting and Immunohistochemistry of P17-39

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Western blot analysis was performed similarly as previously described [25 ]. Briefly, the lysed cell supernatant was run a SDS-PAGE, and blotted with P17- 39, cyclin D1 (MBL), p21 (MBL) and α-Tubulin (MBL) at 0.5∼1 μg/mL, then detected by FITC labeled Goat Anti-mouse secondary antibody (Life). IHC staining was performed as following. In brief, 5 μm thick liver tissues microarray sections were deparaffinized and rehydrated. Antigens retrieval were performed by antigen retrieval solution (BioGenex, San Ramon, CA) with a high pressure cooker for 30 min. Slides were blocked by Goat serum, then blotted with P17-39 at 5 μg/mL. The sections were then incubated with the secondary antibody followed by avidin-biotin-peroxidase complex. The chromogen substrate, NovaRed, was added and finally counter-stained with hematoxylin.

Kang J., Wang J., Cheng J., Cao Z., Chen R., Li H., Liu S., Chen X., Sui J, & Lu F. (2016). Down-regulation of NTCP expression by cyclin D1 in hepatitis B virus-related hepatocellular carcinoma has clinical significance. Oncotarget, 8(34), 56041-56050.

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Immunohistochemical Analysis of Liver Tissue

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The methods are described in detail in the Supporting Information. Liver sections were deparaffinized in EZ‐DeWax (HK 585‐5K; BioGenex, San Ramon, CA) and antigen retrieval was performed in antigen retrieval solution (HK 086‐9K; BioGenex). Slides were treated sequentially with peroxidase suppressor, universal block, and avidin (36000; Thermo Fisher Scientific, Waltham, MA). Slides were incubated sequentially with primary antibody diluted in universal block containing a biotin, biotinylated goat antimouse IgG, and avidin‐biotin complex. Slides were developed with Immpact Nova Red peroxidase substrate (SK‐4805; Vector Labs, Burlingame, CA), counterstained with Mayer’s Hematoxylin (S3309; Agilent Dako, Santa Clara, CA), dehydrated, and mounted in nonaqueous mounting media (H‐5000; VectorLabs). Ki67 was detected with a mouse monoclonal antibody (M7240, MIB‐1; Agilent Dako) at 1:100 dilution and activated caspase 3 was detected with a rabbit antibody (9664S, 5A1E; Cell Signaling Technology, Danvers, MA) at 1:100 dilution.

Chen C.Y., Winer B.Y., Chavez D., Guerra B., Brasky K.M., Eng S., Salas E., Tam D., Simmons J.H., Abee C.R., Delaney W.E., Ploss A., Lanford R.E, & Voitenleitner C. (2020). Woolly Monkey–HBV Infection in Squirrel Monkeys as a Surrogate Nonhuman Primate Model of HBV Infection. Hepatology Communications, 4(3), 371-386.

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Immunofluorescence Analysis of Embryonic Markers

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X-gal staining for detection of β-galactosidase and counterstaining with Nuclear Fast Red were conducted as described previously (Anderson et al., 2004 (link)). For section immunofluorescence, embryos were sectioned, dewaxed, boiled in antigen retrieval solution (Biogenex), and blocked in PBS containing 10% sheep serum and 0.1% Triton X-100. The following primary antibodies were used at 1:100 dilutions in blocking serum: goat anti-ECE1 (R&D AF1784), goat anti-Nrp1 (R&D AF566), goat-anti Nrp2 (R&D AF2215), goat anti-connexin40 (Santa Cruz sc-20466), mouse anti-COUP-TFII (Perseus Proteomics PP-H7147-00), and chicken anti-β-galactosidase (Abcam ab9361). The following secondary antibodies were used: rabbit anti-goat 594 (Invitrogen A11080), rabbit anti-chicken 488 (Jackson ImmunoResearch 303-545-003), and rabbit anti-mouse 594 (Invitrogen A11062). Slides were mounted and photographed as described previously (Rojas et al., 2009 (link)).

Robinson A.S., Materna S.C., Barnes R.M., De Val S., Xu S.M, & Black B.L. (2014). An arterial-specific enhancer of the human Endothelin-converting enzyme 1 (ECE1) gene is synergistically activated by Sox17, FoxC2, and Etv2. Developmental biology, 395(2), 379-389.

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Immunostaining of Paraffin-Embedded Tissues

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Paraffin‐embedded, formalin‐fixed tissue was immunostained for FOXO1 or p‐AMPK or ki‐67 content with primary antibodies against FOXO1 (1:50 dilution) or p‐AMPK (1:100 dilution) or ki‐67 (1:100 dilution). After specimens were deparaffinized in xylene and graded alcohol, epitope retrieval was carried out: sections were heated in a microwave oven at 700 W for 20 min in 1× Antigen Retrieval Solution (Biogenex, San Francisco, USA). Then, endogenous peroxidase was blocked by immersing sections in 0.3% H₂O₂ methanol for 15 min. The reaction was visualized by use of the EnVision Detection Kit (Zhongshan Golden Bridge Biotechnology) with diaminobenzidine tetrahydrochloride as the enzyme substrate. All sections were counterstained with GM hematoxylin staining solution (Zhongshan Golden Bridge Biotechnology). All slides were reviewed independently by two investigators (J.H. and L.H.). Every tumor was given a score reflecting the mean intensity of the staining (0, no staining; 1, low staining; 2, medium staining; and 3, strong staining).21

Zou J., Hong L., Luo C., Li Z., Zhu Y., Huang T., Zhang Y., Yuan H., Hu Y., Wen T., Zhuang W., Cai B., Zhang X., Huang J, & Cheng J. (2016). Metformin inhibits estrogen‐dependent endometrial cancer cell growth by activating the AMPK–FOXO1 signal pathway. Cancer Science, 107(12), 1806-1817.

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