Apc anti mouse il 17a

Madecassic acid (CAS no. 18449-41-7) with a purity of 98.5% was purchased from Jiangsu Yongjian Pharmaceutical Technology Co., Ltd (Taizhou, China). DSS (MW: 36–50 kDa) was purchased from MP Biomedicals Inc. (Irvine, CA, USA). Mouse IL-17 ELISA kit was purchased from Lianke Biotech Co., Ltd (Hangzhou, China). MPO activity assay kit was obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). FITC-anti-mouse CD3, PE-anti-mouse TCR γ/δ, PE-anti-mouse CD4, PE-anti-mouse CD8, and APC-anti-mouse IL-17A were purchased from eBioscience, Inc. (San Diego, CA, USA). Murine IL-1β and IL-23 were purchased from Sino Biological Inc. (Beijing, China). Mouse TCRγ/δ T Cell Isolation Kit was purchased from Miltenyi Biotec Inc. (Bergisch Gladbach, Germany). TRIzol reagent was purchased from SunShine Biotechnology Co., Ltd (Nanjing, China). PPARγ, phosphorylated (p)-PPARγ, PTEN, p-PTEN, PI3K, p-PI3K, Akt, p-Akt, GSK3β, p-GSK3β, mTOR, p-mTOR, NFATc1, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mAb were purchased from HuaBio Inc. (Hangzhou, China). Cyclosporin A, GW9662, MK-2206, and rapamycin were obtained from CSNpharm (Chicago, USA). Rosiglitazone, FK506, LY294002, and SC-97 were purchased from Selleck (Houston, USA).

Intracellular cytokine staining was performed according to the manufacturer's protocol of the Mouse Foxp3 Buffer Set (BD Biosciences) and analyzed using FlowJo software. The following reagents were used: Pacific Blue anti-mouse CD4 (eBioscience), PE anti-mouse TCR-β (BD Biosciences), PE anti-mouse RORγt (eBioscience), FITC anti-mouse IFN-γ (eBioscience), and APC anti-mouse IL-17A (eBioscience).