Log In Sign up
The largest database of trusted experimental protocols

Anti mouse cd19 alexa fluor 647

Manufactured by BioLegend
Visit Supplier

Anti-mouse CD19-Alexa Fluor 647 is a fluorochrome-conjugated antibody that binds to the CD19 antigen expressed on mouse B cells. It is designed for use in flow cytometry applications to identify and enumerate B cell populations.

Automatically generated - may contain errors

Lab products found in correlation

Lsrii flow cytometer, by BD (2 mentions) Facsdiva software, by BD (2 mentions) Cd27 pe, by BioLegend (2 mentions) Igm brilliant violet 421, by BioLegend (2 mentions)

Spelling variants of this product

Anti-CD19 (91 citations) Alexa Fluor 647 (65 citations) Alexa 647 (11 citations) CD19-Alexa Fluor 647 (5 citations) Alexa Fluor® 647 anti-mouse CD19 antibody (3 citations) Alexa Fluor® 647-conjugated anti-mouse CD19 (2 citations)

2 protocols using anti mouse cd19 alexa fluor 647

1

Murine Spleen B Cell Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions from murine spleens (106 cell per test) were stained with fluorochrome-conjugated monoclonal antibodies (mABs) in a single tube containing anti-mouse CD19-Alexa Fluor 647 (BioLegend 115522), IgD-Alexa Fluor 488 (BioLedend, 405718), IgM-Brilliant Violet 421 (BioLegend, 406517) and CD27-PE (BioLegend 124209) antibodies (1:20 dilution) for 30 min at 4°C. Cell-associated fluorescence was assessed with an LSR-II flow cytometer and analyzed using FACSDiva software (both BD). Two independent experiments were conducted, comparing the percentage of CD19+ cells (B cells) from splenocytes between inducible Clec16a knock down mice after tamoxifen treatment and the littermates treated with oil as a control. Data were combined together and B cell percentage was compared via two-sided T-test.
Li J., Jørgensen S.F., Maggadottir S.M., Bakay M., Warnatz K., Glessner J., Pandey R., Salzer U., Schmidt R.E., Perez E., Resnick E., Goldacker S., Buchta M., Witte T., Padyukov L., Videm V., Folseraas T., Atschekzei F., Elder J.T., Nair R.P., Winkelmann J., Gieger C., Nöthen M.M., Büning C., Brand S., Sullivan K.E., Orange J.S., Fevang B., Schreiber S., Lieb W., Aukrust P., Chapel H., Cunningham-Rundles C., Franke A., Karlsen T.H., Grimbacher B., Hakonarson H., Hammarström L, & Ellinghaus E. (2015). Association of CLEC16A with human common variable immunodeficiency disorder and role in murine B cells. Nature communications, 6, 6804.
+ Open protocol
+ Expand
2

Murine Spleen B Cell Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions from murine spleens (106 cell per test) were stained with fluorochrome-conjugated monoclonal antibodies (mABs) in a single tube containing anti-mouse CD19-Alexa Fluor 647 (BioLegend 115522), IgD-Alexa Fluor 488 (BioLedend, 405718), IgM-Brilliant Violet 421 (BioLegend, 406517) and CD27-PE (BioLegend 124209) antibodies (1:20 dilution) for 30 min at 4°C. Cell-associated fluorescence was assessed with an LSR-II flow cytometer and analyzed using FACSDiva software (both BD). Two independent experiments were conducted, comparing the percentage of CD19+ cells (B cells) from splenocytes between inducible Clec16a knock down mice after tamoxifen treatment and the littermates treated with oil as a control. Data were combined together and B cell percentage was compared via two-sided T-test.
Li J., Jørgensen S.F., Maggadottir S.M., Bakay M., Warnatz K., Glessner J., Pandey R., Salzer U., Schmidt R.E., Perez E., Resnick E., Goldacker S., Buchta M., Witte T., Padyukov L., Videm V., Folseraas T., Atschekzei F., Elder J.T., Nair R.P., Winkelmann J., Gieger C., Nöthen M.M., Büning C., Brand S., Sullivan K.E., Orange J.S., Fevang B., Schreiber S., Lieb W., Aukrust P., Chapel H., Cunningham-Rundles C., Franke A., Karlsen T.H., Grimbacher B., Hakonarson H., Hammarström L, & Ellinghaus E. (2015). Association of CLEC16A with human common variable immunodeficiency disorder and role in murine B cells. Nature communications, 6, 6804.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!