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Pcag yfp

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The PCAG-YFP is a plasmid construct that contains the yellow fluorescent protein (YFP) gene under the control of the CAG promoter. The CAG promoter is a synthetic promoter known for driving high levels of gene expression in a wide variety of cell types. This plasmid is commonly used as a reporter for monitoring gene expression or for cellular localization studies.

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Lab products found in correlation

Pcag dsred, by Addgene (4 mentions) Pcalnl dsred, by Addgene (3 mentions) Prl tk, by Promega (3 mentions) Pcag gfp, by Addgene (3 mentions) Pcag cfp, by Addgene (3 mentions) Pcagen, by Addgene (1 mentions) Pcag egfp, by Addgene (1 mentions)

4 protocols using pcag yfp

1

Versatile Fluorescent Protein Expression Plasmids

Cited 2 times
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pCAG-GFP (Addgene plasmid 11150) (Matsuda and Cepko, 2004 (link)). pCAG-YFP (Addgene plasmid 11180) (Matsuda and Cepko, 2004 (link)). pCAG-DsRed (Addgene plasmid 11151) (Matsuda and Cepko, 2004 (link)). pRho-GFP-IRES-AP (referred to as Rho-GFP) (Emerson and Cepko, 2011 (link)). pCAG-nlacZ (Cepko lab, Harvard Medical School) pCAGEN (Addgene plasmid 11160) (Matsuda and Cepko, 2004 (link)). pCALNL-DsRed (Addgene plasmid 13769) (Matsuda and Cepko, 2004 (link)). pCAFNF-DsRed (Addgene plasmid 13771). (Matsuda and Cepko, 2004 (link)). pCALNL-luc2 (Tang et al., 2015 (link)). pRL-TK (#E2241; Promega, Madison, WI).
Tang J.C., Drokhlyansky E., Etemad B., Rudolph S., Guo B., Wang S., Ellis E.G., Li J.Z, & Cepko C.L. (2016). Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies. eLife, 5, e15312.
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2

Diverse Fluorescent Protein Expression Vectors

Cited 1 time
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pCAG-GFP (Addgene plasmid 11150) 39 (link), pCAG-YFP (Addgene plasmid 11180) 39 (link), pCAG-CFP (Addgene plasmid 11179) 39 (link), pCAG-tdT (Cepko lab, Harvard Medical School), pCAG-mCherry (Cepko lab, Harvard Medical School), pCAG-DsRed (Addgene plasmid 11151) 39 (link), pRL-TK (Promega, #E2241), pRho-GFP-IRES-AP (referred to as Rho-GFP in main text) 40 (link), pCAG-nlacZ (Cepko lab, Harvard Medical School). pAAV-CAG-FLEX-tdT was a gift from Edward Boyden (Addgene plasmid #28306), pCALNL-DsRed (Addgene plasmid #13769) 41 (link).
Tang J.C., Rudolph S., Dhande O.S., Abraira V.E., Choi S., Lapan S., Drew I.R., Drokhlyansky E., Huberman A.D., Regehr W.G, & Cepko C.L. (2015). Cell Type-Specific Manipulation with GFP-Dependent Cre Recombinase. Nature neuroscience, 18(9), 1334-1341.
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3

Fluorescent Plasmid Validation Protocol

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Plasmids encoding fluorescent reporter proteins were used to validate electroporation: pCAG‐DsRed (Addgene: 11151), pCAG‐YFP (Addgene: 11180), pCAG‐EGFP (Addgene: 11150), pCAG‐CFP (Addgene: 11179), pCBA‐TdTomato (Addgene: 28017). Plasmids were amplified and purified according to the suppliers’ recommendations, filtered, and stored at −20°C until use.
Dempsey B., Turner A.J., Le S., Sun Q., Bou Farah L., Allen A.M., Goodchild A.K, & McMullan S. (2015). Recording, labeling, and transfection of single neurons in deep brain structures. Physiological Reports, 3(1), e12246.
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4

Diverse Fluorescent Protein Expression Vectors

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
pCAG-GFP (Addgene plasmid 11150) 39 (link), pCAG-YFP (Addgene plasmid 11180) 39 (link), pCAG-CFP (Addgene plasmid 11179) 39 (link), pCAG-tdT (Cepko lab, Harvard Medical School), pCAG-mCherry (Cepko lab, Harvard Medical School), pCAG-DsRed (Addgene plasmid 11151) 39 (link), pRL-TK (Promega, #E2241), pRho-GFP-IRES-AP (referred to as Rho-GFP in main text) 40 (link), pCAG-nlacZ (Cepko lab, Harvard Medical School). pAAV-CAG-FLEX-tdT was a gift from Edward Boyden (Addgene plasmid #28306), pCALNL-DsRed (Addgene plasmid #13769) 41 (link).
Tang J.C., Rudolph S., Dhande O.S., Abraira V.E., Choi S., Lapan S., Drew I.R., Drokhlyansky E., Huberman A.D., Regehr W.G, & Cepko C.L. (2015). Cell Type-Specific Manipulation with GFP-Dependent Cre Recombinase. Nature neuroscience, 18(9), 1334-1341.
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