Log In Sign up
The largest database of trusted experimental protocols

Mouse anti mcherry

Manufactured by Takara Bio
Visit Supplier
Sourced in Japan

Mouse anti-mCherry is a monoclonal antibody that specifically recognizes the mCherry fluorescent protein. mCherry is a red fluorescent protein derived from the Discosoma species of coral. The mouse anti-mCherry antibody can be used to detect and monitor the expression of mCherry-tagged proteins in various applications.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit anti dsred, by Takara Bio (3 mentions) Chicken anti gfp, by Abcam (3 mentions) Rabbit anti mcherry, by Abcam (2 mentions) Rabbit anti phospho akt ser473, by Cell Signaling Technology (2 mentions) Vt1000, by Leica camera (2 mentions) Vectashield h 1000, by Vector Laboratories (2 mentions) Rabbit anti phospho s6 s235 236, by Cell Signaling Technology (2 mentions) Alexa fluor 488 conjugated goat anti chicken igg, by Jackson ImmunoResearch (2 mentions) Alexa fluor 647 conjugated goat anti rabbit igg, by Jackson ImmunoResearch (2 mentions) Cy3 conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (2 mentions) Cy3 conjugated donkey anti mouse igg, by Jackson ImmunoResearch (2 mentions) Chicken anti egfp, by Abcam (1 mentions) Goat anti chicken alexa fluor 488 secondary, by Thermo Fisher Scientific (1 mentions) Fv1000 confocal system, by Olympus (1 mentions) Mouse anti gfp, by Roche (1 mentions) Rabbit anti gfp, by Abcam (1 mentions) Goat anti mouse, by LI COR (1 mentions) To pro 3, by Thermo Fisher Scientific (1 mentions) Rabbit anti egfp, by Thermo Fisher Scientific (1 mentions) Z0334, by Merck Group (1 mentions)

14 protocols using mouse anti mcherry

1

Fluorescent Immunohistochemistry of Embryos

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Embryos were fixed in 4% paraformaldehyde solution in PBS at 4°C overnight, stored at −20°C in methanol and stained according to a previously described fluorescence immunohistochemistry protocol (Turner et al., 2014 (link)). The following antibodies were all used at a dilution of 1/500: mouse anti-mCherry (Clontech) with goat anti-mouse Alexa-Fluor®-568 secondary (Invitrogen); chicken anti-EGFP (Abcam) with goat anti-chicken Alexa-Fluor®-488 secondary (Invitrogen) (Du et al., 2017 (link); Hall et al., 2014 (link)).
Britto D.D., Wyroba B., Chen W., Lockwood R.A., Tran K.B., Shepherd P.R., Hall C.J., Crosier K.E., Crosier P.S, & Astin J.W. (2018). Macrophages enhance Vegfa-driven angiogenesis in an embryonic zebrafish tumour xenograft model. Disease Models & Mechanisms, 11(12), dmm035998.
+ Open protocol
+ Expand
2

Immunostaining of Fluorescently-Labeled Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
We incubated free-floating coronal sections (50 µm) (n=3) in PB supplemented with 4% BSA and 0.3% Triton X-100 for 1 h. We then incubated the sections with cocktails of primary antibodies (mouse anti-mCherry [1:500, Clontech Laboratories, 632543; RRID: AB_2307319] + guinea pig anti-vGluT1 [1:500, Frontier Institute, vGluT1-GP-Af570; RRID: AB_2571534]) overnight at 4°C. After rinsing 3×10 min in PB, we incubated the sections in a cocktail of the corresponding fluorescence secondary antibodies (Alexa Fluor 488– anti–guinea pig [706-545-148 RRID: AB_2340472] + Alexa Fluor 594–anti-mouse [715-586-151 RRID: AB_2340858] Jackson Immunoresearch Laboratories, 1:100 dilution) for 2 h at room temperature. After rinsing, we mounted sections on slides. We collected fluorescent images with an Olympus FV1000 Confocal System (Olympus). We took images sequentially with different lasers with 10X or 100X oil immersion objectives and we collected z-axis stacks at 0.2 µm. We successfully repeated this procedure three times.
Venniro M., Caprioli D., Zhang M., Whitaker L.R., Zhang S., Warren B.L., Cifani C., Marchant N.J., Yizhar O., Bossert J.M., Chiamulera C., Morales M, & Shaham Y. (2017). The anterior insular cortex→central amygdala glutamatergic pathway is critical to relapse after contingency management. Neuron, 96(2), 414-427.e8.
+ Open protocol
+ Expand
3

Antibodies for Cellular Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were obtained from commercial sources: mouse-anti-mCherry (Clontech), rabbit-anti-GFP (Abcam, ab290), mouse-anti-GFP (Roche, 11814460001), rabbit-anti-Rab8 (D22D8) (Cell Signaling Technology, #697), and rabbit anti-ELKS (Proteintech, 22211-1-AP). Polyclonal rabbit-anti-MICAL3 antibody were custom raised and has been used previously20 (link). Secondary antibodies were purchased from commercial sources: IRDye 800CW/680LT goat anti-rabbit, and goat anti-mouse (Li-Cor Biosciences).
Liu Q., Remmelzwaal S., Heck A.J., Akhmanova A, & Liu F. (2017). Facilitating identification of minimal protein binding domains by cross-linking mass spectrometry. Scientific Reports, 7, 13453.
+ Open protocol
+ Expand
4

Immunofluorescence Staining of Larval Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence was carried out as previously described with some modifications64 . Larvae were incubated in proteinase k (10 μg/ml) for 30 min at room temperature for permeabilization before fixation in 4% paraformaldehyde. The following primary and secondary antibodies were used: mouse anti-mCherry (1:500/Clontech) with goat anti-mouse Alexa Fluor 568 (1:500/Invitrogen) and rabbit anti-DsRed (1:500/Clontech) with goat anti-rabbit Alexa Fluor 568 (1:500/Invitrogen).
Du L.Y., Darroch H., Keerthisinghe P., Ashimbayeva E., Astin J.W., Crosier K.E., Crosier P.S., Warman G., Cheeseman J, & Hall C.J. (2017). The innate immune cell response to bacterial infection in larval zebrafish is light-regulated. Scientific Reports, 7, 12657.
+ Open protocol
+ Expand
5

Immunohistochemistry for Protein Localization

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry was performed as previously described54 (link). 3,3-Diaminobenzidine (Sigma) stains were performed using an ABC labeling kit (Vector Laboratories, Burlingame, CA). Primary antibodies were chosen according to previous studies in our lab or reports in the literature or instructions from the vendors: Rabbit anti-P-Smad2 (1:1000; Chemicon, AB3849), rabbit anti-eGFP (1:500; Life Technologies, A11122), chicken anti-eGFP (1:1000, Avis, GFP-1020), mouse anti-mCherry (1:200, Clontech, 632543), chicken anti-Tbr2 (1:500, Millipore, AB15894), mouse anti-MCM2 (1:500, BD Biosciences, 610700), rat anti-BrdU (1:1000; Abcam, AB6326), goat anti-DCX (1:500; Santa Cruz Biotechnology, sc-8066), mouse anti-PCNA (1:200; DAKO, M0879), rabbit anti-GFAP (1:1000; DAKO, Z0334), mouse anti-NeuN (1:1000; Millipore, MAB377), goat anti-Sox2 (1:200, Santa Cluz Biotechnology, sc-17320), mouse anti-HA (1:1000; Covance, MMS-101P), rabbit anti-c-fos (1:10000; Millipore, PC38). Antigen retrieval with 3 mol/L HCl was used for BrdU. For fluorescent stains, secondary antibodies were purchased from either Molecular Probes or Jackson Immunoresearch. For fluorescent staining procedures, final washes included the nuclear stain Topro-3 (1:500; Molecular Probes) or DAPI (5 μg/mL, Sigma) for 0.5 h at room temperature.
He Y., Zhang H., Yung A., Villeda S.A., Jaeger P.A., Olayiwola O., Fainberg N, & Wyss-Coray T. (2014). ALK5-dependent TGF-β signaling is a major determinant of late stage adult neurogenesis. Nature neuroscience, 17(7), 943-952.
+ Open protocol
+ Expand
6

Immunohistochemistry for mCherry, Sox2, and GFP

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry was performed as described previously17 (link). In brief, sections on glass slides were treated in 10 mM Tris–HCl pH 9.0, 1 mM EDTA at 95 °C for one minute, incubated with the blocking buffer (10% normal donkey serum, 0.1% Triton-X100 in PBS), incubated with the primary antibodies at 4 °C overnight, washed, incubated with the secondary antibodies at room temperature for 1 h, and washed. The following antibodies were used; mouse anti-mCherry (#632543, Clontech), goat anti-Sox2 (AF2018, R&D Systems), mouse anti-GFP (#11814460001, Sigma), anti-mouse IgG conjugated to Alexa 594 (A21203, Molecular Probe) and anti-mouse IgG conjugated to Alexa 488 (A21202, Molecular Probe), anti-goat IgG conjugated to Alexa 594 (A11058, Molecular Probe). Hoechst 33342 (H1399, Molecular Probe) was used for nuclear staining.
Naruse M, & Saito T. (2022). Immediate protein expression from exogenous mRNAs in embryonic brain. Scientific Reports, 12, 17145.
+ Open protocol
+ Expand
7

Antibodies Used for Neuronal Immunostaining

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used for immunostaining included: mouse anti-nc82 (1:50, Developmental Studies Hybridoma Bank), rat-anti-Chinmo (1:500; Wu et al., 2012 (link)), rabbit anti-Br-Z3 (1:250; this study), rabbit anti-GFP (1:1000; Invitrogen), chicken anti-GFP (1:500; Rockland Immunochemicals), rabbit anti-dsRed (1:500; Clontech), mouse anti-mCherry (1:500; Clontech), rabbit anti-β-gal (1:500; MP Biomedicals), chicken anti-β-gal (1:500; Abcam). Most primary antibodies were preabsorbed against fixed embryos. Secondary goat antibodies were conjugated to Alexa Fluor 488, 568 or 633 (Molecular Probes). Slides were mounted in Vectashield (Vector Labs) or dehydrated through an ethanol series and mounted in DPX. Images were collected on a Leica SP5 (Light Microscopy Imaging Center, Indiana University). Confocal stacks were merged using Leica LSM software. Samples that were directly compared were prepared under identical conditions and imaged in parallel.
Wu Y.C., Chawla G, & Sokol N. (2020). let-7-Complex MicroRNAs Regulate Broad-Z3, Which Together with Chinmo Maintains Adult Lineage Neurons in an Immature State. G3: Genes|Genomes|Genetics, 10(4), 1393-1401.
+ Open protocol
+ Expand
8

Immunofluorescence Antibody Dilutions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Alexa Fluor 488-conjugated goat anti-mouse IgG (Life Technologies) was diluted 1:500, mouse anti-myc antibody (9E10, Roche) was diluted 1:500 and mouse anti-mCherry (Clontech) was diluted 1:250.
Rampon C., Gauron C., Lin T., Meda F., Dupont E., Cosson A., Ipendey E., Frerot A., Aujard I., Le Saux T., Bensimon D., Jullien L., Volovitch M., Vriz S, & Joliot A. (2015). Control of brain patterning by Engrailed paracrine transfer: a new function of the Pbx interaction domain. Development (Cambridge, England), 142(10), 1840-1849.
+ Open protocol
+ Expand
9

Cryosectioning and Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Preparation of tissue for cryosectioning and immunofluorescence were performed as described.4 (link) 12 μm cryosections were obtained with a Cryostat HM560. Primary antibodies used were: chicken anti-GFP (Abcam, ab13970) at 1:2000, mouse anti-mCherry (Clontech, 632543) at 1:450, rabbit anti-dsRed (Clontech, 632496) at 1:300, mouse anti-chondroitin sulfate (Sigma, C8035) at 1:300, rabbit anti-Laminin (Sigma, L9393) at 1:200, rat anti-BrdU (Novus Bio, NB500-169) at 1:300. Secondary antibodies used were: goat anti-chicken-Alexa 488 (Thermo Fisher, A-11039), goat anti-mouse-Alexa 555 (Thermo Fisher, A-21424), goat anti-rabbit-Alexa 555 (Thermo Fisher, A-21428), goat anti-mouse-Alexa 633 (Thermo Fisher, A-21046), goat anti-rat-Alexa 633 (Thermo Fisher, A-21094) at 1:1000.
Cudak N., López-Delgado A.C., Rost F., Kurth T., Lesche M., Reinhardt S., Dahl A., Rulands S, & Knopf F. (2024). Compartmentalization and synergy of osteoblasts drive bone formation in the regenerating fin. iScience, 27(2), 108841.
+ Open protocol
+ Expand
10

Immunohistochemical Labeling of Brain Tissues

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were administered a xylazine and ketamine cocktail (10 mg and 100 mg per kg body weight, respectively) intraperitoneally and were transcardially perfused with 8–10 ml of 0.9% saline, followed by 8–10 ml of 4% paraformaldehyde in PBS. Following extraction, brains were placed in 4% paraformaldehyde in PBS overnight, before being placed in 30% sucrose in PBS for 5–7 d in preparation for sectioning. Then, 40-μm coronal sections were prepared using a freezing microtome and tissue was labeled with the following antibodies: chicken anti-GFP (1:500 dilution; Aves Labs, GFP-1020), mouse anti-mCherry (1:500 dilution; Clontech, 632543), rabbit anti-cFos (1:500 dilution; Synaptic Systems, 226 003), Alexa-488 donkey anti-chicken (1:250 dilution; Jackson Immuno Research, 703-545-155), Cy3 donkey anti-rabbit (1:250 dilution; Jackson Immuno Research, 711-165-152) and Cy3 donkey anti-mouse (1:250 dilution; Jackson Immuno Research, 715-165-151). For secondary and primary incubations, antibody blocking was performed using normal horse serum (Sigma-Aldrich, H0146). Antibody amplification was used to visualize ChR2-mCherry and ChR2-EYFP. Images were taken at ×20 magnification using an Olympus VS120 slide scanner18 (link).
Cook J.R., Li H., Nguyen B., Huang H.H., Mahdavian P., Kirchgessner M.A., Strassmann P., Engelhardt M., Callaway E.M, & Jin X. (2022). Secondary auditory cortex mediates a sensorimotor mechanism for action timing. Nature neuroscience, 25(3), 330-344.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!