Functional mitochondria isolation kit

Cerebral cortical mitochondria were isolated by differential centrifugation using a functional mitochondria isolation kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), which was mentioned in our previous study [29 (link)]. The isolated mitochondria were stored at −70 °C.

The mitoKATP channel agonist (DZX), antagonist (5-HD), and GJ inhibitor (CBX) were obtained from Sigma (St. Louis, MO, USA); PKC agonist (PMA) and PKC antagonist (Ro-31-8425) were obtained from Millpore (St. Louis, MO, USA); Cx43 (ab11369), p-Cx43 (ab30559), β-actin (ab8227) , GAPDH (ab8245) and VDAC-1 (ab34726) antibodies were obtained from Abcam (Cambridge, MA, USA); PKCε (sc-214) and p-PKCε (sc-12355) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); horseradish peroxidase-conjugated secondary antibodies were obtained from Bioss (bs-0293G-HRP, bs-0296G-HRP, Beijing, China); 2,3,5-triphenyltetrazolium chloride (TTC) were obtained from Sigma (St. Louis, MO, USA); In situ Cell Death Detection Kit was obtained from Roche Molecular Biochemicals (Mannheim, Germany); The SOD activity kit, MDA content kit, functional mitochondria isolation kit and mitochondrial protein extraction kit were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).