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Micro slide 4 well plates

Manufactured by Ibidi
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Micro slide 4 well plates are a type of laboratory equipment designed to hold small samples or specimens for microscopic observation. These plates feature four individual wells, allowing for the simultaneous examination of multiple samples. The plates are made of high-quality materials and are suitable for a variety of cell culture and tissue analysis applications.

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Lab products found in correlation

C11440 orca flash 4, by Hamamatsu Photonics (2 mentions) Elements analysis 5, by Nikon (2 mentions) Alexa fluor 568, by Thermo Fisher Scientific (2 mentions) Janelia fluor 646, by Novus Biologicals (2 mentions) Eclipse ti2 microscope, by Nikon (2 mentions)

Close spelling variants of this product

ยต-Slide 4 Well Micro-slides

2 protocols using micro slide 4 well plates

1

Super-Resolution Imaging of INTS1 and RPB1

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells (4x103) were plated onto micro slide 4 well plates (Ibidi), fixed in 4% formaldehyde, and immunostained with appropriate primary and secondary antibodies. Imaging experiments were carried out with a Nikon eclipse Ti2 microscope equipped with Nikon Instruments (NSTORM). For two color imaging dSTORM imaging, Janelia Fluor 646 [Novus Bio NBP1-7-2739JF646 Lot #36481-051615] was used as a secondary staining for INTS1 and Alexa Fluor 568 [Invitrogen A11077 Lot #1917936] was used as a secondary staining for RPB1 Tyr-1. The secondary antibodies were used with MEA STORM imaging buffer and were imaged continuously with 5000 frames collected per filter range at a frequency of 30 ms. The images were acquired using a 100x, 1.49 NA objective, and imaged onto a Hamamatsu C11440 ORCA-flash 4.0. Storm analysis was carried out with Nikon Elements Analysis 5.02.01 for identification of molecules. Molecule list files were then exported from Nikon elements to be further analyzed using software Clus-Doc software in MATLAB R2018b (Pageon et al., 2016 (link)). Cluster density analysis, specifically DBSCAN function, was carried out after manually selecting region of interest corresponding to the nucleus. Statistical significance and graphing were performed using Prism software.
Beckedorff F., Blumenthal E., daSilva L.F., Aoi Y., Cingaram P.R., Yue J., Zhang A., Dokaneheifard S., Valencia M.G., Gaidosh G., Shilatifard A, & Shiekhattar R. (2020). The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts. Cell reports, 32(3), 107917.
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2

Super-Resolution Imaging of INTS1 and RPB1

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells (4x103) were plated onto micro slide 4 well plates (Ibidi), fixed in 4% formaldehyde, and immunostained with appropriate primary and secondary antibodies. Imaging experiments were carried out with a Nikon eclipse Ti2 microscope equipped with Nikon Instruments (NSTORM). For two color imaging dSTORM imaging, Janelia Fluor 646 [Novus Bio NBP1-7-2739JF646 Lot #36481-051615] was used as a secondary staining for INTS1 and Alexa Fluor 568 [Invitrogen A11077 Lot #1917936] was used as a secondary staining for RPB1 Tyr-1. The secondary antibodies were used with MEA STORM imaging buffer and were imaged continuously with 5000 frames collected per filter range at a frequency of 30 ms. The images were acquired using a 100x, 1.49 NA objective, and imaged onto a Hamamatsu C11440 ORCA-flash 4.0. Storm analysis was carried out with Nikon Elements Analysis 5.02.01 for identification of molecules. Molecule list files were then exported from Nikon elements to be further analyzed using software Clus-Doc software in MATLAB R2018b (Pageon et al., 2016 (link)). Cluster density analysis, specifically DBSCAN function, was carried out after manually selecting region of interest corresponding to the nucleus. Statistical significance and graphing were performed using Prism software.
Beckedorff F., Blumenthal E., daSilva L.F., Aoi Y., Cingaram P.R., Yue J., Zhang A., Dokaneheifard S., Valencia M.G., Gaidosh G., Shilatifard A, & Shiekhattar R. (2020). The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts. Cell reports, 32(3), 107917.
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