Nf light assay kits

Manufactured by Quanterix

Sourced in United States

The NF-light assay kits from Quanterix are designed to detect and quantify neurofilament light chain (NF-L) levels in biological samples. NF-L is a well-established biomarker that can provide insights into neuronal injury and neurodegeneration. The kits utilize Quanterix's proprietary Single Molecule Array (Simoa) technology to achieve high-sensitivity and precise NF-L measurements.

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Lab products found in correlation

Simoa hd 1 analyser, by Quanterix (3 mentions) Lumipulse g1200, by Fujirebio (2 mentions) Hd x analyzer, by Quanterix (1 mentions) Tau 2, by Quanterix (1 mentions)

Close spelling variants of this product

NF-Light kit (13 citations) NF-Light (11 citations) NF-Light assay (8 citations)

5 protocols using nf light assay kits

Measurements of Alzheimer's Biomarkers

Cited 2 times

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CSF and blood samples from each participant were collected at a single session at approximately 8 am following overnight fasting as previously described.^{5 (link),30 (link)} Concentrations of CSF Aβ40, Aβ42, t-tau, and p-tau181 were measured by chemiluminescent enzyme immunoassay using a fully automated platform (LUMIPULSE G1200; Fujirebio). CSF NfL was measured via commercial ELISA kit (UMAN Diagnostics). Plasma Aβ42 and Aβ40 were measured in the C2N Diagnostics commercial laboratory with the PrecivityAD immunoprecipitation–mass spectrometry assay.^{16 (link)} Plasma p-tau181 and p-tau231 were measured in the Clinical Neurochemistry Laboratory, University of Gothenburg, using in-house Single molecule array (Simoa) assays on an HD-X analyzer (Quanterix), as previously described.^{19 (link),23 (link)} Plasma NfL was measured with Quanterix Nf-Light assay kits at Washington University on a HD-X analyzer. All assays were performed by personnel who were blind to participant information.

Schindler S.E., Karikari T.K., Ashton N.J., Henson R.L., Yarasheski K.E., West T., Meyer M.R., Kirmess K.M., Li Y., Saef B., Moulder K.L., Bradford D., Fagan A.M., Gordon B.A., Benzinger T.L., Balls-Berry J., Bateman R.J., Xiong C., Zetterberg H., Blennow K, & Morris J.C. (2022). Effect of Race on Prediction of Brain Amyloidosis by Plasma Aβ42/Aβ40, Phosphorylated Tau, and Neurofilament Light. Neurology, 99(3), e245-e257.

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Plasma Tau and NfL Quantification

Cited 1 time

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Tau and NfL concentrations were measured in plasma using the Simoa HD-1 Analyser and the “tau-2.0” and “NF-light” assay kits (Quanterix), according to manufacturer’s instructions and as described previously [18 (link)]. Samples from each diagnostic group were assorted across different loading plates and assay runs. Samples were tested in duplicate, by taking two aliquots from the same plate well. Quality control parameters are detailed in the Supplementary Material. All “tau-2.0” testing kits were from a single batch. “NF-light” kits were taken from two separate batches due to a manufacturer issue: a full calibration sample set tested for each batch showed no significant variation between them.

Thompson A.G., Anastasiadis P., Druyeh R., Whitworth I., Nayak A., Nihat A., Mok T.H., Rudge P., Wadsworth J.D., Rohrer J., Schott J.M., Heslegrave A., Zetterberg H., Collinge J., Jackson G.S, & Mead S. (2021). Evaluation of plasma tau and neurofilament light chain biomarkers in a 12-year clinical cohort of human prion diseases. Molecular Psychiatry, 26(10), 5955-5966.

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Biomarker Profiling in Alzheimer's Disease

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CSF and blood samples were collected at approximately 8 am following overnight fasting as previously described.⁸, ³⁹ Plasma Aβ42 and Aβ40 were measured in the C2N Diagnostics commercial laboratory with an immunoprecipitation‐mass spectrometry assay (St. Louis, MO, USA).⁴⁰ Plasma NfL was measured with Quanterix Nf‐Light assay kits on an HD‐X analyzer. Concentrations of CSF Aβ40, Aβ42, total tau (t‐tau), and tau phosphorylated at 181 (p‐tau181) were measured by chemiluminescent enzyme immunoassay using a fully automated platform (LUMIPULSE G1200, Fujirebio, Malvern, PA, USA). CSF NfL was measured via commercial ELISA kit (UMAN Diagnostics, Umeå, Sweden). APOE genotype was determined by genotyping rs7412 and rs429358 with Taqman genotyping technology.⁴¹

Aschenbrenner A.J., Li Y., Henson R.L., Volluz K., Hassenstab J., Verghese P., West T., Meyer M.R., Kirmess K.M., Fagan A.M., Xiong C., Holtzman D., Morris J.C., Bateman R.J, & Schindler S.E. (2022). Comparison of plasma and CSF biomarkers in predicting cognitive decline. Annals of Clinical and Translational Neurology, 9(11), 1739-1751.

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Plasma Biomarker Quantification Protocol

Cited 1 time

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Tau and NfL concentrations were measured in plasma using the Simoa HD-1 Analyser and the “tau-2.0” and “NF-light” assay kits (Quanterix), according to manufacturer’s instructions and as described previously^{18 (link)}. Samples from each diagnostic group were assorted across different loading plates and assay runs. Samples were tested in duplicate, by taking two aliquots from the same plate well. Quality control parameters are detailed in the Supplementary Material. All “tau-2.0” testing kits were from a single batch. “NF-light” kits were taken from 2 separate batches due to a manufacturer issue: a full calibration sample set tested for each batch showed no significant variation between them.

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Plasma Biomarkers for Neurodegeneration

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Tau and NfL concentrations were measured in plasma using the Simoa HD-1 Analyser and the "tau-2.0" and "NF-light" assay kits (Quanterix), according to manufacturer's instructions and as described previously 18 . Samples from each diagnostic group were assorted across different loading plates and assay runs. Samples were tested in duplicate, by taking two aliquots from the same plate well. For quality control, data were reviewed after testing and samples without two valid results or with a coefficient of variation (CV) over 15% between duplicates were re-tested. All "tau-2.0" testing kits were from a single batch. "NF-light" kits were taken from 2 separate batches due to a manufacturer issue: a full calibration sample set tested for each batch showed no significant variation between them.

Thompson A.G., Anastasiadis P., Druyeh R., Whitworth I., Nayak A., Nihat A., Mok T.H., Rudge P., Wadsworth J.D.F., Rohrer J.D., Schott J.M., Heslegrave A., Zetterberg H., Collinge J., Jackson G.S., & Mead S. (2020). Evaluation of plasma tau and neurofilament light chain biomarkers in a 12-year clinical cohort of human prion diseases.

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