Epsilon 1 6d

DNA of the fungal cultures was prepared as previously reported (Libert et al. 2015 (link)). Briefly, after the incubation time, 300 mg of wet sample were transferred to cryotubes containing 0.25 ml of acid-washed glass beads (Sigma Aldrich, Diegem, Belgium) and put at −80 °C during 40 min and freeze-dried overnight with a freeze-dryer Epsilon 1-6D (Martin Christ, Osterode am Harz, Germany). Freeze-dried fungi were then beat-beaten with a Mini bead beater (Biospec Products, OK, USA) during 1 min at maximal speed.
Subsequently, the total DNA was extracted with an adapted phenol chloroform (24:1) protocol (Ashktorab and Cohen, 1992 (link)) and purified with the Qiagen CTAB genomic Tip-20 kit (Qiagen Benelux—B.V., KJ Venlo, The Netherlands) according to the manufacturer’s protocol. A 100-μl Gibco® DNase, RNase, protease free water (Life Technologies, Gent, Belgium) was used to elute the DNA. The DNA integrity was verified on a 2 % agarose gel. The DNA concentration and purity were evaluated with a Nanodrop® 2000 (Thermo Scientific, Wilmington, USA).

The fungal strains were grown in a S10 Sabouraud liquid medium (Bio-Rad, Temse, Belgium) at 25 °C with constant agitation between 3 and 10 days according to the species’ growth conditions.
After this incubation time, 300 mg of wet sample was transferred to cryotubes containing 0.25 ml of acid-washed glass beads (Sigma-Aldrich, Diegem, Belgium) put at −80 °C during 40 min and freeze-dried overnight with a freeze-dryer Epsilon 1-6D (Martin Christ, Osterode am Harz, Germany). Freeze-dried fungi were subsequently beat-beaten with a Mini bead beater (Biospec Products, OK, USA) during 1 min at maximal speed.
The total DNA was extracted with an adapted phenol chloroform (24:1) protocol (Ashktorab and Cohen 1992 (link)) and purified with the Qiagen CTAB genomic Tip-20 kit (Qiagen Benelux, B.V., KJ Venlo, the Netherlands) according to the manufacturer’s recommendation. DNA was eluted with 100 μl Gibco® DNase, RNase, protease free water (Life Technologies, Gent, Belgium). The DNA integrity was verified on a 2 % agarose gel. The DNA amount and purity were evaluated with a Nanodrop® 2000 (Thermo Scientific, Wilmington, USA).