T4 polymerase

Dulbecco's Modified Eagle's Medium (DMEM), Opti-MEM® I Reduced-Serum Medium, fetal bovine serum (FBS), TRIzol RNA isolation reagent, ElectroMAX DH12S competent cells with M13KO7 helper phage, and primers were obtained from Invitrogen (Grand Island, NY or Shanghai, China). E3330, myrecitin, synthetic siRNA against Pol β and other general chemicals were obtained from Sigma-Aldrich (St, Louis, MO, USA). Biotin-conjugated or unconjugated tetrahydrofuran sites containing oligonucleotides were obtained from Takara (Dalian, China). The VEGF ELISA kit was obtained from R&D (Minneapolis, MN, USA). Dual luciferase vector pmirGLO, the Dual-Glo® luciferase assay kit, the FuGENE 6 transfection reagent, T4 polynucleotide kinase (T4 PNK), T4 ligase, T4 polymerase, restriction endonucleases, and high-fidelity Pfu DNA polymerase were purchased from Promega (Madison, WI, USA). The QuickChange mutagenesis kit was purchased from Stratagene (Santa Clara, CA, USA). The Halt protease inhibitor cocktail, Protein A/G agarose beads, a GST protein interaction pulldown kit, LightShift chemiluminescent EMSA kit, SuperSignal West Pico chemiluminescent reagents, horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG antibodies and other Western blot-related reagents were purchased from Pierce (Rockford, IL, USA).