Tumor pieces or cell pellets were lysed in RIPA buffer (Sigma-Aldrich) with protease inhibitor and phosphatase inhibitor cocktail (Roche), resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes. Antibodies used were as follows: phosphorylated Ser1981 ATM (ab81292), phosphorylated Ser127 YAP (ab76252) (Abcam); CTGF (bs-0743R, Bioss); caspase 3 (#9662), Asp175 cleaved caspase 3 (#9664), GAPDH (#5174), phosphorylated Ser139 H2A.X (#9718), PARP (#9542), Asp214 cleaved PARP (#9541) (Cell Signaling); β-actin (sc-47778), survivin (sc-17779), YAP (sc-15407) (Santa Cruz), Ki67 (RM9106S1, Thermo Scientific), α-tubulin (04-1117, Millipore). Apoptosis evaluation through Western blot analysis was performed on both floating and adherent cells.
Phosphorylated ser1981 atm
Manufactured by Abcam
Phosphorylated Ser1981 ATM is a laboratory product that detects the phosphorylation of the ATM (Ataxia Telangiectasia Mutated) protein at serine 1981. ATM is a critical protein involved in the cellular response to DNA damage. Phosphorylation of ATM at serine 1981 is an important marker of ATM activation.
Automatically generated - may contain errors
Lab products found in correlation
Protease inhibitor and phosphatase inhibitor cocktail, by Roche (2 mentions)
α tubulin, by Merck Group (2 mentions)
Caspase 3, by Bioss Antibodies (2 mentions)
Phosphorylated ser127 yap ab76252, by Abcam (2 mentions)
Ctgf bs 0743r, by Bioss Antibodies (2 mentions)
Cleaved caspase 3 asp175, by Cell Signaling Technology (2 mentions)
Phosphorylated ser139 h2a x, by Cell Signaling Technology (2 mentions)
Cleaved parp asp214, by Cell Signaling Technology (2 mentions)
Gapdh, by Cell Signaling Technology (2 mentions)
Ripa buffer, by Merck Group (2 mentions)
2 protocols using phosphorylated ser1981 atm
1
Apoptosis Protein Analysis in Cancer Cells
2
Apoptosis Protein Analysis in Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tumor pieces or cell pellets were lysed in RIPA buffer (Sigma-Aldrich) with protease inhibitor and phosphatase inhibitor cocktail (Roche), resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes. Antibodies used were as follows: phosphorylated Ser1981 ATM (ab81292), phosphorylated Ser127 YAP (ab76252) (Abcam); CTGF (bs-0743R, Bioss); caspase 3 (#9662), Asp175 cleaved caspase 3 (#9664), GAPDH (#5174), phosphorylated Ser139 H2A.X (#9718), PARP (#9542), Asp214 cleaved PARP (#9541) (Cell Signaling); β-actin (sc-47778), survivin (sc-17779), YAP (sc-15407) (Santa Cruz), Ki67 (RM9106S1, Thermo Scientific), α-tubulin (04-1117, Millipore). Apoptosis evaluation through Western blot analysis was performed on both floating and adherent cells.
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