Confocal laser scanning microscope model tc sp5 mo tandem

Samples from stocks and scions were analyzed on a Leica confocal laser scanning microscope (model TC-SP5/MO-TANDEM) using a krypton/argon with a laser excitation fluorescence/emission of 488/525 nm for green fluorescence and 580 nm/665 nm emission. All images were recorded and analyzed with Leica Las AF software, followed by processing using Photoshop 8.0 software (Adobe) as described (Xoconostle-Cázares et al., 1999 (link)).

Leaves from Citrus plants transformed with the CsPP16-β-defensin-GFP or CsPP16-magainin-GFP fusion construct were collected, sectioned manually employing a surgical blade on a glass slide, to obtain the main vein and transversally sectioned in slices of 1 mm. Sections were submerged in water and analyzed immediately with a Leica confocal laser-scanning microscope (model TC-SP5/MO-TANDEM) at the LanSE facility in CINVESTAV-Mexico City, using a krypton/argon laser and the following filter settings: 488 nm excitation and 525 nm emission for green fluorescence, and 580/665 nm for chlorophyll autofluorescence. All images were recorded and analyzed with Leica Las AF software, followed by processing with Photoshop 8.0 software (Adobe), as described previously (Xoconostle-Cázares et al., 1999 (link)).

Transgenic plants expressing the GFP fusion constructs were analyzed with a Leica confocal laser-scanning microscope (model TC-SP5/MO-TANDEM) using a krypton/argon laser and the following filter settings: 488 nm excitation and 525 nm emission for green fluorescence, and 580/665 nm for chlorophyll autofluorescence. All images were recorded and analyzed with Leica Las AF software, followed by processing with Photoshop 8.0 software (Adobe) as described (Xoconostle-Cázares et al., 1999 (link)).