For the microbiological analysis, samples of 5 g of disinfected larvae were collected and diluted tenfold in physiological peptone solution (PPS, 0.85% NaCl, 0.01% peptone) before pulverizing the larvae in the solution by using an ethanol sterilized home type mixer (Bosch CNHR 25). Similarly, 5 g of substrate was sampled from each replicate and diluted 1:10 in PPS. Next, both larval and substrate samples were homogenized using a stomacher (BagMixer 400CC, Interscience, France) for 1 min. For each sample, the total aerobic viable count and the specific Salmonella count were determined. All plate counts were performed according to the ISO standards for microbial analyses of food and feed as compiled by Dijk et al. (2015) . For the total aerobic viable count, serial dilutions were made in PPS, plated on Plate Count Agar (PCA, Biokar Diagnostics, Beauvais, France), and incubated at 30 °C for 72 h. Salmonella was counted by plating the diluted samples on a chromogenic RAPID′ Salmonella agar (BioRad Laboratories, Belgium) and incubating the plates at 37 °C for 24 h. The cell density of all inocula was also verified by plating a serial dilution on both the RAPID’ Salmonella agar and PCA.
Cnhr 25
Manufactured by Bosch
Sourced in Belgium
The CNHR 25 is a lab equipment product from Bosch. It is a compact and versatile device designed for various laboratory applications. The core function of the CNHR 25 is to provide precise temperature and humidity control for samples or experiments within a controlled environment.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using cnhr 25
1
Microbiological Analysis of Disinfected Larvae
2
Edible Insect Microbiome and Viruses
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For experiment 1 (identification of bacterial endospores in edible insects), four samples of living yellow mealworms (Tenebrio molitor) and four samples of living house crickets (Acheta domesticus) were collected at the end of their rearing cycle. For each insect species, two different industrial rearing companies in Belgium (yellow mealworms) or Belgium and the Netherlands (house crickets) were sampled two times each (2 samples x 2 rearers x 2 sampling moments or batches). For experiment 2 (detection of foodborne viruses in edible insects), the same 17 mealworm and cricket samples that were investigated previously 7 for their bacterial composition (yellow mealworm, house cricket and tropical house cricket (Gryllodes sigillatus) samples from different rearing companies and batches) were employed, as well as an extra sample of lesser mealworms (Alphitobius diaperinus) obtained in another study 4 where it was used to study microbial dynamics during rearing (larvae day 35, post-harvest). After removal of dead specimens, insects were sedated by cooling them for approximately 1 h at 4 °C and subsequently, the samples were homogenised by pulverisation with a sterilised hand-held mixer (Bosch CNHR 25, Belgium) as described earlier 9 .
3
Insect Preparation for Consumption
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Samples of fully grown and living insects ready for consumption were transported to the laboratory at ambient temperature and immediately processed upon arrival. Prior to analysis, insects were sedated by cooling (± 4 °C, 1 h). Subsequently, three subsamples of 30 g were taken aseptically from each batch and pulverized (Bosch CNHR 25, max speed) as described previously (Stoops et al., 2016) (link).
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