The chemical reagents ABTS ((2, 5, 7, Tween 40, and phenolic compound standards (gallic acid, catechin, chlorogenic acid, caffeic acid, ferulic acid, vanillin, quercetin, were obtained from Sigma-Aldrich (Saint Louis, Missouri, USA). methanol (HPLC grade) was obtained from J-TBacker (Alfragide, Lisbon, Portugal). In turn, the Folin-Ciocalteu reagent was obtained from Dinâmica (Indaiatuba, São Paulo, Brazil). Acetone, acetic acid, gallic acid, linoleic acid, methanol, and chloroform were obtained from Vetec (Saint Louis, Missouri, USA), while sulfuric acid was obtained from Merck (Darmstadt, Hessen, Germany). Ethanol, was obtained from Synth (Diadema, São Paulo, Brazil), while n-hexane and potassium persulfate were obtained from Neon (São Paulo, São Paulo, Brazil). All reagents were analytical grade, and stock and buffer solutions were prepared using distilled water.
Chloroform
Manufactured by Vetec
Sourced in Brazil
Chloroform is a colorless, volatile liquid chemical compound. It is commonly used as a solvent in various laboratory and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Quercetin, by Merck Group (3 mentions)
Methanol, by Vetec (3 mentions)
Gallic acid, by Vetec (3 mentions)
Hydrochloric acid (hcl), by Vetec (3 mentions)
Ethanol, by Vetec (3 mentions)
Catechin, by Merck Group (2 mentions)
Ferulic acid, by Merck Group (2 mentions)
Vanillin, by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Acetone, by Vetec (2 mentions)
Polyvinyl alcohol (pva), by Merck Group (2 mentions)
Sulfuric acid, by Merck Group (1 mentions)
Caffeic acid, by Merck Group (1 mentions)
Chlorogenic acid, by Merck Group (1 mentions)
Abts 2 5 7, by Merck Group (1 mentions)
Linoleic acid, by Vetec (1 mentions)
Folin ciocalteu reagent, by Dinâmica (1 mentions)
Acetic acid, by Vetec (1 mentions)
Sodium phosphate dibasic, by Vetec (1 mentions)
Methanol, by Merck Group (1 mentions)
11 protocols using chloroform
1
Antioxidant Characterization of Phenolic Compounds
2
Poly(vinyl alcohol)-based Hydrogel Formulation
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Poly (vinyl alcohol) (98% hydrolysis degree, Mw 13,000–23,000 g/mol) and methanol came from Sigma-Aldrich®, St. Louis, MO, USA. Poly(lactic acid) (Mw 200,000 g/mol) was a donation from Natureworks, Minnetonka, MN, USA. Dimethylsulfoxide, glutaraldehyde (aqueous solution 25% (v/v)), sodium chloride (Pro Analyses), dibasic sodium phosphate (P.A.) and chloroform were purchased from Vetec® Química Fina, Brazil. Potassium chloride (P.A.) was procured from Isofar, Brazil. Monobasic potassium phosphate (P.A.) was from Reagen, Brazil. Anforicin B (the amphotericin micellar system) was from Cristália (Itapira, São Paulo, Brazil), and amphotericin B drug powder (AmB) was purchased from Indofine Chemical Company, Inc. (Hillsborough, NJ, USA).
3
Cardiac Troponin T Immunosensor Development
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Human cardiac troponin T (cTnT) antigen and mouse monoclonal anti-cTnT antibody were purchased from Calbiochem (Darmstadt, Germany). Pyrrole-2-carboxylic acid (COOH-Py) (99%), nanoclay (montmorillonite clay base material) surface modified with 0.5–5 wt.% aminopropyltriethoxysilane and 15–35 wt.% octadecylamine (NH2-NCY), potassium ferricyanide (K3[Fe(CN)6]), potassium ferrocyanide (K4[Fe(CN)6]), acetonitrile (anhydrous, 99.8%), glutaraldehyde (GA) (50%, w/w), N-hydroxysuccinimide (NHS), N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide (EDC), and glycine (Gly) were obtained from Sigma-Aldrich (St. Louis, USA). Lithium perchlorate (LiClO4) and chloroform were purchased from Vetec (Rio de Janeiro, Brazil). Phosphate buffer saline (PBS) solution (0.01 mol L−1, pH 7.4) was used in all experiments for dilution of the samples. Ultrapure water was obtained from a Millipore water purification system (18 MΩ cm−1, Milli-Q) from Millipore (St. Louis, Missouri, USA). All the chemical reagents used to prepare buffers and solutions were of analytical grade.
4
Phytochemical Standard Compounds Acquisition
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The standards of luteolinidin chloride, gallic acid and catechin hydrate were obtained from Sigma-Aldrich (St. Louis, MO, USA). The apigeninidin chloride was obtained from Chromadex (Santa Ana, CA, USA). The analytic grade reagents acetone, chloroform, methanol, and hydrochloric acid were purchased from VETEC (São Paulo, Brazil) and vanillin, Folin-Ciocalteu and ethanolamine from Sigma-Aldrich (St. Louis, MO, USA). High performance liquid chromatography (HPLC) grade reagents (acetic acid, acetone, acetonitrile, ethyl acetate, hexane, isopropyl alcohol, methyl alcohol, and formic acid) were purchased from Tedia (São Paulo, Brazil).
5
Fabrication of PLGA-based Tea Tree Oil Delivery
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Medical grade PLGA copolymer (82:18 molar ratio, inherent viscosity midpoint of 1.8 dlg−1) was purchased from Corbion Purac (PURASORB® PLG 8218, Amsterdam, Netherlands). Australian Melaleuca alternifolia (tea tree) essential oil (Desert Essence, Hauppauge, NY, USA) and furan-2(5H)-one were purchased from Sigma-Aldrich (St. Louis, MO, USA). Chloroform (Vetec, Duque de Caxians, Brazil) and N,N-dimethylformamide (DMF) (LabSynth, Diadema, Brazil) were used as solvent. All solvents were of analytical grade.
6
Antioxidant Compound Extraction Protocol
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Sodium erythorbate (SE), potassium acetate, ethanol, ethylenediaminetetraacetic acid (EDTA), thiobarbituric acid (TBA) and chloroform were obtained from Vetec (Sao Paulo, Brazil). Quercetin, rutin, ferulic acid, coumaric acid, pinocembrin, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′- azino-bis (3-ethylbenzothiazoline-6-sulfphonic acid), TPTZ (2,4,6-Tris(2-pyridyl)-s-triazine), Folin–Ciocalteu phenol reagents, Trolox, β-carotene/linoleic acid, were obtained from Sigma–Aldrich (Sternheim, Germany).
7
Characterization of Polypropylene Contamination
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In this work, polypropylene was used in pellet form (Prism 2400) supplied by Braskem S.A, Brazil. It presents a melt flow index (MFI) of 20 g/10 min (ASTM 1238, 230 °C, 2.16 kg) and a density of 0.902 g/cm3 (ASTM D 792). The chemicals used for contamination were chloroform (Vetec, 99.8%, CAS No. 67663), toluene (Vetec, 99.5%, CAS No. 108883), benzophenone (Acros Organics, 99%, CAS No. 119619), tetracosane (Merck, 99%, CAS No. 646311) and heptane (Synth, 99%, CAS No. 1422825). Ethanol (Merck, 99%) and acetic acid (Merck, 99%) were employed to prepare the food simulants, with 10% (v/v) ethanol and 3% (w/v) acetic acid respectively in MiliQ water.
8
Cell Culture and Cytotoxicity Assay
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Roswell Park Memorial Institute 1640 (RPMI 1640) culture medium and foetal bovine serum (FBS) were purchased from Gibco Invitrogen (Karlsruhe, Germany). Penicillin/streptomycin, dimethyl sulphoxide (DMSO) and salt 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were purchased from Sigma Chemical (St. Louis, MO, USA). Folin-Ciocalteu reagent and 2,2-diphenyl-1-picryhydrazyl (DPPH) were purchased from Merck KGaA (Darmstadt, Germany). Methanol, hydrochloric acid, ethanol, ferric chloride, sodium hydroxide, chloroform, anhydrous sodium sulphate, sulphuric acid, sodium carbonate, gallic acid and ethyl acetate were purchased from Vetec Química Fina Ltda. (Duque de Caxias, RJ, Brazil).
9
Polymer-based Composite Synthesis
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Holmium chloride hexahydrate, poly(vinyl alcohol) (MM 13,000–23,000 g mol−1, 98% hydrolyzed), poly(3-hydroxy-butyrate-co-3-hydroxy-valerate) (PHB-HV) with 5% or 8% amounts of 3-hydroxy-valerate in copolymer were obtained from the Sigma-Aldrich. Acetylacetonate and chloroform were from Vetec (Brazil). Ammonium hydroxide and nitric acid were obtained from commercial suppliers and used as obtained.
10
Antioxidant Compounds Characterization
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2,4-Dinitrophenylhydrazine (DNPH), 1,1-Diphenyl-2-picrylhydrazyl (DPPH), butylated hydroxyltoluene (BHT), 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid (ABTS), 6-hydroxy-2,5,7,8-tetramethylchroman-2carboxylic acid (Trolox), quercetin, catechin, and β-carotene were obtained from Sigma Chemical Co. (MO, USA). Potassium persulfate, tween 40, Folin-Ciocalteau, sodium carbonate from Dinamina, sulfuric acid, methanol, ethanol, hydrochloric acid, ascorbic acid, chloroform, linolenic acid, gallic acid, aluminum chloride, sodium acetate, and vanillin were obtained from Vetec (RJ, Brazil).
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