Cycleave human aldh2 typing probe primer set

The mutation of Glu504 Lys of the ALDH2 gene was detected and classified among the JCV-positive samples utilizing real time-PCR. Fluorescence melting curve analysis was performed using a LightCycler (Roche Diagnostics GmbH, Mannheim, Germany) with primers and probes obtained from Takara Bio Inc. (TaKaRa Cycleave Human ALDH2 Typing Probe/Primer Set).
PCR conditions were as follows: initially at 95 °C for 10 s, followed by 60 cycles of thermal shift at 95 °C for 5 s, annealing at 53 °C for 10 s, and extended at 72 °C for 20 s. The fluorescence emitted was measured during this process.

A total of 108 JCV-positive samples were analyzed by real-time PCR, and the G-to-A substitution rs671 (i.e., the Glu504Lys or Glu487Lys polymorphism) in ALDH2 was detected and classified. A fluorescence melting curve analysis was performed using a LightCycler (Roche Diagnostics GmbH, Mannheim, Germany) with primers and probes obtained from Takara Bio Inc. (TaKaRa Cycleave Human ALDH2 Typing Probe/Primer Set).
PCR conditions were as follows: 95 °C for 10 sec, followed by 60 cycles of denaturation at 95 °C for 5 sec, annealing at 53 °C for 10 sec, and extension at 72 °C for 20 sec. Fluorescence was measured during this process.
To confirm results of the above-mentioned method, we also analyzed the whole samples using the PCR-CTPP method as described^{36 (link)}.