Recombinant BMP-2 was purchased from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-GAPDH monoclonal antibody (#2118) was obtained from Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-Runx2 (sc-10758) and anti-His-probe (sc-803) polyclonal antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Rabbit anti-Hey1 polyclonal antibody (ab22614) was purchased from Abcam (Cambridge, MA, USA). HRP-conjugated goat secondary antibody (AP307P) was obtained from Millipore (Billerica, MA, USA). Alexa Fluor® 594, 488-conjugated secondary antibodies (A11037 and A27034) and the pcDNA3.1 vector were obtained from Invitrogen (Carlsbad, CA, USA).
Recombinant bmp 2
Manufactured by Merck Group
Sourced in United States
Recombinant BMP-2 is a laboratory-produced growth factor that is structurally and functionally similar to the naturally occurring Bone Morphogenetic Protein 2 (BMP-2). BMP-2 is a key signaling molecule involved in bone and cartilage formation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using recombinant bmp 2
1
Characterization of Recombinant BMP-2 Signaling
2
In Vivo Bone Regeneration with Chitosan-BMP2
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Animal experiment was approved by Institutional Animal Care and Use Committee of Zhejiang Chinese Medical University, and performed by strictly following the Chinese Law on animal experimentation. Fifteen Male beagle dogs (5–6 months old, 10.3–12.5 kg), were provided by the Experimental Animal Center, Zhejiang Chinese Medicine University, China (Number of qualitative qualification SCXK (Su) 2010–0002). The following reagents were used: Chitosan, (molecular weight 190k-220k, degree of deacetylation > 90%; Sigma, USA); sodium tripolyphosphate (STPP; Sigma, USA); recombinant BMP-2 (Sigma, USA); ELISA kit (R&D, USA); lyophilizer (Labconco Co., USA); enzyme-linked immunosorbent assay (ELISA); microplate reader (Thermo Scientific Varioskan Flash); micro-CT (Skyscan Bruker, Belgium); scanning electron microscope (SEM; S-3000N Hitachi, Japan); sputter coater (Hitachi E-1010); material testing system (Instron 5569, USA).
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