Lc 20a spectrophotometer

Analytical HPLC was performed on an Agilent 1200 with an Agilent DAD spectrophotometer (Agilent Technologies, Santa Clara, USA) and a YMC-Pack Pro C18 (5 μm, 4.6 × 250 mm, YMC Ltd., Japan). Preparative HPLC was performed on a Shimadzu LC-20A spectrophotometer and a YMC-Pack Pro C18 column (5 μm, 20 × 250 mm). NMR (1D and 2D) spectra were recorded by a Bruker AVANCE III 600 spectrometer. The ESI-MS spectra were measured on a 6430 Triple Quad mass spectrometer (Agilent Technologies, Santa Clara, USA). The HR-ESI-MS spectra were recorded using a Q-TOF mass spectrometer (Waters Corporation, Milford, USA). UV and IR data were measured using a JASCO V-550 UV/vis and a JASCO FT/IR-480 plus spectrometers (Jasco, Japan), respectively. Normal phase silica-gel (200–300 mesh) was purchased from Qingdao Haiyang Chemical Co., Ltd., Octadecylsilanized silica (ODS) gel (50 μm) was purchased from YMC Ltd. in Japan. The optical density was measured on a Tecan Infinite®200 PRO microplate reader (Tecan, Swiss).

The ESI-MS spectra were recorded on a 6430 Triple Quad mass spectrometer (Agilent Technologies, Santa Clara, USA). The HR-ESI-MS spectra were obtained on a Micromass Q-TOF mass spectrometer (Waters Corporation, Milford, USA). 1D and 2D NMR spectra were measured on a Bruker AVANCE III 600 spectrometer. UV data were recorded using a JASCO V-550 UV/vis spectrometer (Jasco International Co., Ltd., Tokyo, Japan). IR data were recorded on a JASCO FT/IR-480 plus spectrometer (Jasco International Co., Ltd., Tokyo, Japan). Optical rotations were measured on a JASCO P1020 digital polarimeter (Jasco International Co., Ltd., Tokyo, Japan). Analytical HPLC was performed on an Agilent 1100 with an Agilent DAD spectrophotometer and a YMC-Pack Pro C18 (5 μm, 4.6 × 250 mm), while preparative HPLC was performed on an Shimadzu LC-20A spectrophotometer and a YMC-Pack Pro C18 column (5 μm, 20 × 250 mm). Normal phase silica gel (200–300 mesh, Qingdao Haiyang Chemical Co., Ltd.) and octadecylsilanized (ODS) silica gel (50 μm, YMC Ltd., Japan) were also used for column chromatography (CC).