Quantitative measurement of Zonulin was carried out by using the commercially available IDK® Zonulin ELISA Kit (Immundiagnostik AG, Bensheim, Germany).
Idk zonulin elisa kit
Manufactured by Immundiagnostik
Sourced in Germany
The IDK® Zonulin ELISA Kit is a quantitative in vitro diagnostic test used for the measurement of zonulin levels in human serum and plasma samples. Zonulin is a protein involved in the regulation of intestinal permeability. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to determine the concentration of zonulin in the provided samples.
Automatically generated - may contain errors
6 protocols using idk zonulin elisa kit
1
Quantitative Zonulin Measurement by ELISA
2
Zonulin as Intestinal Permeability Marker
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zonulin concentrations were assessed in stool as marker for intestinal permeability using the IDK® Zonulin ELISA kit (Immundiagnostik AG, Bensheim, Germany).
3
Metabolic and Inflammatory Biomarkers Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fasting serum levels of glucose, insulin, creatinine, uric acid, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and gamma-glutamyl transferase (GGT), triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) were determined by an automatic biochemical analyzer (ILAB650, Instrumentation Laboratory and TOSO AIA 900, Italy), while the high-density lipoprotein cholesterol (HDL-C) concentration was estimated using the Friedewald formula20 (link). The homeostatic model assessment (HOMA) index was calculated with the formula HOMA-IR = [fasting glucose (mg/dL) × insulin (IU)/405]. Serum zonulin levels were quantified using IDK® Zonulin ELISA Kit (Immundiagnostik AG, Germany). C-reactive protein (CRP) was quantified using the Quantikine Human C-reactive protein ELISA kit (R&D Systems cat# DCRP00), TNF-α using the Quantikine high sensitivity Human TNF-α ELISA kit (R&D Systems cat# HSTA00E) and IL-6 using the Quantikine high sensitivity Human IL-6 ELISA kit (R&D Systems cat# HS600B). Intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were quantified in serum samples with an ELISA kit (Booster® from Vinci Biochem S.r.l., Vinci, Italy). The Cockcroft-Gault equation was used for estimating creatinine clearance (CG index).
4
Assessing Gut Permeability and Zonulin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On arrival on each examination day, participants emptied their bladder. Urine was collected for 4 hours after the standardised breakfast and the lactulose-mannitol containing drink. Urine was stored in the fridge during collection, pooled, mixed and aliquoted into 2.0 mL tubes and stored at −80°C. The percentage of excreted lactulose and mannitol in urine was measured as described in online supplementary materials . Gut permeability was estimated based on the ratio of urinary lactulose and mannitol. In addition, fasting serum concentrations of zonulin, a suggested biomarker of gut barrier function,25 (link) were measured using IDK Zonulin ELISA kit (Immundiagnostik AG, Bensheim, Germany). The lower limit of detection was 0.22 ng/mL.
5
Quantification of Serum Immunoglobulin and Inflammatory Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total serum IgG was measured using the IgG human uncoated ELISA Kit from Invitrogen, Cat: 88-50550, following the manufacturer’s instructions using a serum dilution of 1:100,000. Serum calprotectin was quantified using the RayBio Human S100-A8/A9 complex ELISA Kit from RayBiotech, Cat: ELH-S100A8-9, following the manufacturer’s instructions. Serum zonulin was measured using the IDK Zonulin ELISA Kit from Immundiagnostik AG, Cat: K5601, following the manufacturer’s instructions.
6
Plasma Endotoxin and Zonulin Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma samples were collected for LPS measurement using the PYROGENT™-5000 and Kinetic-QCL™ assays (Lonza Walkersville, Inc. Walkersville, MD, USA). The endotoxin concentrations in the plasma samples were determined from a standard curve. Plasma zonulin levels were measured using the IDK Zonulin ELISA Kit (Immundiagnostik AG, Salem, NH, USA), according to the manufacturer’s instructions. The assay was based on the competitive ELISA method. A biotinylated zonulin tracer was used as a competitor. Free zonulin in the sample competes with the tracer for binding to anti-zonulin antibodies immobilized on the microtiter plate wells. The standards and controls were assayed simultaneously with the samples.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!