Polyclonal antibodies specific

Manufactured by Cell Signaling Technology

Sourced in United States

Polyclonal antibodies specific are laboratory reagents used for the detection and analysis of target proteins in various applications, such as Western blotting, immunohistochemistry, and ELISA. These antibodies are produced by immunizing animals with the target antigen, resulting in a mixture of antibodies that recognize different epitopes on the protein of interest.

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Lab products found in correlation

Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (2 mentions) Lsm780 confocal laser scanning microscope, by Zeiss (1 mentions) Alexa fluor secondary antibody, by Thermo Fisher Scientific (1 mentions) Immun blot pvdf membrane, by Bio-Rad (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) β actin antibody, by Bioss Antibodies (1 mentions) Thiazolyl blue tetrazolium bromide, by Merck Group (1 mentions) Taurine, by Merck Group (1 mentions) Uo126, by Merck Group (1 mentions) Nox4 antibody, by Abcam (1 mentions) Purified recombinant mouse netrin 1, by R&D Systems (1 mentions) Anti actin antibody, by Merck Group (1 mentions) Anti β actin monoclonal antibody, by Merck Group (1 mentions) Monoclonal anti ki 67 antibody, by Agilent Technologies (1 mentions) Caspase 6, by Santa Cruz Biotechnology (1 mentions) Caspase 8, by Santa Cruz Biotechnology (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) Caspase 7, by Santa Cruz Biotechnology (1 mentions) P21waf1, by Santa Cruz Biotechnology (1 mentions) Polyclonal antibodies against cyclin d1, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Phospho-specific p44/42 MAPK mouse and rabbit polyclonal antibodies Specific antibodies Polyclonal antibodies

7 protocols using polyclonal antibodies specific

Visualizing EphA2-Liprin-α1 Colocalization

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Colocalization between EphA2 and liprinα–1 was assessed by confocal microscopy analysis performed as described [30 (link)]. Serum-starved T24 cells plated on four-well chamber slides (Nunc, Thermo Fisher Scientific) were stimulated with progranulin (150 nm for 30 min). After treatment, cells were rinsed twice with DPBS (Thermo Fisher Scientific) and fixed in 4% (wt/vol) paraformaldehyde for 30 min. After washing, EphA2 and liprinα–1 were detected using specific polyclonal antibodies from Cell Signaling Technology followed by immunoreaction with Alexa-Fluor secondary antibodies (Thermo Fisher). Confocal analysis was performed using a 63 ×, 1.3 oil-immersion objective of an LSM-780 confocal laser-scanning microscope (ZEISS) with filters set at 450/594 nm for dual-channel imaging. All images were analyzed using ImageJ (National Institute of Health) and Adobe Photoshop CS6 (Adobe Systems). Images are representative of 10 independent fields from three independent experiments.

Buraschi S., Neill T., Xu S.Q., Palladino C., Belfiore A., Iozzo R.V, & Morrione A. (2020). Progranulin/EphA2 axis: A novel oncogenic mechanism in bladder cancer. Matrix biology : journal of the International Society for Matrix Biology, 93, 10-24.

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Western blot analysis of autophagy and MAPK signaling

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After treatment with rapamycin or trehalose, 2 × 10⁶ BV2 microglial cells were harvested by centrifugation at indicated time points. After washing with PBS, cells were lysed using sample buffer. Subsequently, cells lysates were sonicated and boiled. Western blotting of LC3B, Beclin-1, p-p38, p38, p-ERK, ERK and β-actin was performed according to the manufacturer’s instruction using specific polyclonal antibodies (Cell Signaling Technology). Briefly, proteins were electrophoresed on 12% SDS-PAGE gel under reducing conditions and transferred on to Immun-Blot PVDF Membrane (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 5% nonfat milk and 0.1% Tween-20 in TBS for 2 h at room temperature and then were incubated with primary antibodies overnight at 4 °C. Then, membranes were incubated with a infrared fluorescent dye (IRDye, LI-COR Biosciences, Lincoln, Nebraska, USA) for 1 h and 30 min at RT and protein bands were detected with an Odyssey Infrared Imaging System (LI-COR Biosciences).

Bussi C., Ramos J.M., Arroyo D.S., Gaviglio E.A., Gallea J.I., Wang J.M., Celej M.S, & Iribarren P. (2017). Autophagy down regulates pro-inflammatory mediators in BV2 microglial cells and rescues both LPS and alpha-synuclein induced neuronal cell death. Scientific Reports, 7, 43153.

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AMPK Protein Level Quantification

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AMPK and phosphorylated AMPK (Thr 172) protein levels were determined in cell lysates by employing specific polyclonal antibodies (Cell Signaling Technology Inc.). The β‐actin antibody was from Bioss Antibodies Inc..

Giacco A., delli Paoli G., Simiele R., Caterino M., Ruoppolo M., Bloch W., Kraaij R., Uitterlinden A.G., Santillo A., Senese R., Cioffi F., Silvestri E., Iervolino S., Lombardi A., Moreno M., Goglia F., Lanni A, & de Lange P. (2020). Exercise with food withdrawal at thermoneutrality impacts fuel use, the microbiome, AMPK phosphorylation, muscle fibers, and thyroid hormone levels in rats. Physiological Reports, 8(3), e14354.

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Ginsenoside Effects on BDNF Signaling

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Ginsenosides Re, Rf, Rg1, and Rh1 were provided by Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China). Thiazolyl blue tetrazolium bromide (MTT), taurine, and CORT were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). A monoclonal anti-BDNF antibody was purchased from Abcam (Cambridge, UK). Polyclonal antibodies specific for ERK, JNK, p38, and phospho-p38, as well as a monoclonal antibody specific for phospho-ERK were obtained from Cell Signaling Technology (Danvers, MA, USA). Monoclonal antibodies specific for α-tubulin and phospho-JNK were acquired from Santa Cruz Biotechnology (Dallas, TX, USA). PD98059, SB203580, and SP600125 were purchased from Calbiochem (La Jolla, CA, USA).

Lee W.J., Lee G.H., Hur J., Lee H.G., Kim E., Won J.P., Cho Y., Choi M.J, & Seo H.G. (2020). Taurine and Ginsenoside Rf Induce BDNF Expression in SH-SY5Y Cells: A Potential Role of BDNF in Corticosterone-Triggered Cellular Damage. Molecules, 25(12), 2819.

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Netrin-1 Cardioprotective Mechanisms

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Purified recombinant mouse netrin-1 (R&D Systems, Minneapolis, MN, USA) was administered at doses ranging from 1 to 50 μg/kg, dissolved in normal saline, via injection into the LV lumen at the onset of reperfusion. Some mice were subjected to tail vein injection with netrin-1 (5 μg/kg) also immediately at the onset of reperfusion. UO126, a MEK1/2 inhibitor (200 μg/kg, dissolved in DMSO<0.01%), and PTIO (1 mg/kg, dissolved in normal saline), a specific NO chelator, were purchased from Sigma-Aldrich in highest purity (St. Louis, MO, USA), and administered via injection into the LV lumen at the onset of reperfusion.
Polyclonal antibodies specific for phosphorylated and total forms of ERK1/2, eNOS, were obtained from Cell Signaling Technology (CST, Danvers, MA, USA). NOX4 antibody was purchase from Abcam (Abcam, Cambridge, MA, USA). Anti-actin, antibody, and other chemicals in highest purity, were purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA).

Bouhidel J.O., Wang P., Siu K.L., Li H., Youn J.Y, & Cai H. (2014). Netrin-1 Improves post-Injury Cardiac Function in vivo via DCC/NO-dependent Preservation of Mitochondrial Integrity, While Attenuating Autophagy. Biochimica et biophysica acta, 1852(2), 277-289.

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Antibodies for Apoptosis and Proliferation

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Monoclonal antibodies specific to cleaved caspase-9, cleaved caspase-8, cleaved caspase-3, and cleaved PARP, as well as polyclonal antibodies specific to MMP-2 and MMP-9 were obtained from Cell Signaling Technology (Beverly, MA, USA). The monoclonal anti-β-actin antibody was purchased from Sigma (St. Louis, MO, USA) and the monoclonal anti-Ki-67 antibody was purchased from Dako (Produktionsvej, Glostrup, Denmark).

Sugano H., Shirai Y., Horiuchi T., Saito N., Shimada Y., Eto K., Uwagawa T., Ohashi T, & Yanaga K. (2018). Nafamostat Mesilate Enhances the Radiosensitivity and Reduces the Radiation-Induced Invasive Ability of Colorectal Cancer Cells. Cancers, 10(10), 386.

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Immunoblotting Analysis of Cellular Signaling

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Polyclonal antibodies specific to extracellular signal regulated kinase (ERK), phospho-ERK, p38MAPK, phospho-p38MAPK, c-Jun N-terminal kinase (JNK), phospho-JNK, AKT, and phospho-AKT were obtained from Cell Signaling (Danvers, MA, USA). U0126, SP600125, SB203580, and LY294002 were obtained from Calbiochem (San Diego, CA, USA). Polyclonal antibodies against cyclin D1, cyclin E, CDK2, CDK4, p53, p21WAF1, p27KIP1, and GAPDH were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Polyclonal antibodies specific to FAS, B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), X-linked inhibitor of apoptosis protein (XIAP), poly(ADP-ribose) polymerase-1 (PARP-1), caspase-3, caspase-6, caspase-7, caspase-8, caspase-9, and actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and Cell Signaling (Danvers, MA, USA). Polyclonal antibodies to phospho-endothelial nitric oxide synthase (eNOS) (S1177) and eNOS were obtained from Cell Signaling (Danvers, MA, USA). Human recombinant VEGF was purchased from Research and Diagnostic Systems, Inc. (Minneapolis, MN, USA).

Song J.H., Park J., Park S.L., Hwang B., Kim W.J., Lee C, & Moon S.K. (2021). A Novel Cyclic Pentadepsipeptide, N-Methylsansalvamide, Suppresses Angiogenic Responses and Exhibits Antitumor Efficacy against Bladder Cancer. Cancers, 13(2), 191.

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