SK-UT-1, SK-UT-1B, and MES-SA cells were purchased from American Type Culture Collection. SK-LMS-1 cells were provided by Sebastian Bauer (West German Cancer Center, Essen). Cell line identity and purity were verified using the Multiplex Cell Authentication and Contamination Tests (Multiplexion). All cell lines were regularly tested for mycoplasma contamination using the Venor GeM Mycoplasma Detection Kit (Minerva). Cell lines were cultured as follows: SK-LMS-1 in RPMI-1640 (Life Technologies), 15% FBS; SK-UT-1 and SK-UT-1B in MEM (Life Technologies), 10% FBS; MES-SA in McCoy’s medium, 10% FBS. All media were supplemented with 1% penicillin/streptomycin and 1% L-glutamine (Biochrom).
Mes sa
Manufactured by Thermo Fisher Scientific
The MES-SA is a laboratory equipment product designed for sample analysis. It provides core functionality for sample handling and measurement, but a detailed description cannot be provided while maintaining an unbiased and factual approach. Further information about the intended use or specific capabilities of the MES-SA is not available.
Automatically generated - may contain errors
Lab products found in correlation
Mes sa, by American Type Culture Collection (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Harvard Bioscience (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Sk ut 1b, by American Type Culture Collection (1 mentions)
Sk ut 1, by American Type Culture Collection (1 mentions)
L glutamine, by Harvard Bioscience (1 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Dmem media, by Thermo Fisher Scientific (1 mentions)
Rpmi media, by Thermo Fisher Scientific (1 mentions)
Mes sa dx5, by American Type Culture Collection (1 mentions)
2 protocols using mes sa
1
Cell Line Authentication and Cultivation
2
Characterization of Human Cancer Cell Lines
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The human uterine sarcoma cell lines MES-SA and the doxorubicin selected MES-SA/Dx5 were obtained from ATCC (MES-SA: No. CRL-1976™, MES-SA/Dx5: No. CRL-1977™). The human mammary carcinoma cell lines MCF7, T47D, MDA-MB-231, MDA-MB-468, Hs578T, BT-549, the mouse leukemic P388 and its doxorubicin selected subline P388/ADR were obtained from the National Cancer Institute's Developmental Therapeutics Program (National Institutes of Health). P388/ADR, and Dx5 cells were maintained in 800 and 500 nmol/L doxorubicin (Adriamycin), respectively. The human breast cancer and the mouse leukemia cell lines were cultured in RPMI media (Life Technologies) supplemented with 10% fetal bovine serum, 5 mmol/L glutamine, and 50 units/mL penicillin and streptomycin (Life Technologies). MES-SA and MES-SA/Dx5 cells were cultured in supplemented DMEM media (Life Technologies). The chicken DT40 B cell line was grown in RPMI-1640 medium supplemented with 7% fetal bovine serum, 3% chicken serum, 50 μM 2-mercaptoethanol and penicillin/streptomycin. Wild-type DT40 clone18 cells [44] and a BRCA1 null mutant line [45] were used. All cell lines were cultured at 37 °C, 5% CO2.
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