Cystatin C, capture antibody and labeled antibody were provided by the FMMU (Fourth Military Medical University). Rhodamine 6G, Aminopropyl Triethoxysilane (APTES), Ethylene Glycol (EG), Tetraethoxysilane (TEOS), Fluorescein and Bis (2,4,6-trichlorophenyl), Cetyltrimethyl Ammonium Bromide (CTAB), Oxalate (TCPO), Methyl Trimethoxy Silane (MTMS) and other chemical reagents were purchased from Sangon Biotech (Shanghai, CHINA). The 96-well transparent microtiter plates were obtained from Corning Incorporated (Corning-Costar, Corning, NY).
The concentration of the coating solution is 0.05 mol/L carbonate buffer, pH 9.6. It contains 2.93 g NaHCO3 and 1.59 g Na2CO3 per liter. The PBS buffer (pH 7.4) was prepared by dissolving 8.0 g NaCl, 2.9 g Na2HPO4, 0.2 g KH2PO4, and 0.2 g KCl in 1 L of water. The 96-well plate was washed by PBST (PBS solution containing 0.05% Tween 20) solution. Cystatin C antigen and antibody were diluted with PBSB (PBS containing 0.1% BSA) solution as blocking solution. The PBS buffer (pH 7.1) was used to covalently immobilize monoclonal antibodies on nanoparticles.
The concentration of the coating solution is 0.05 mol/L carbonate buffer, pH 9.6. It contains 2.93 g NaHCO3 and 1.59 g Na2CO3 per liter. The PBS buffer (pH 7.4) was prepared by dissolving 8.0 g NaCl, 2.9 g Na2HPO4, 0.2 g KH2PO4, and 0.2 g KCl in 1 L of water. The 96-well plate was washed by PBST (PBS solution containing 0.05% Tween 20) solution. Cystatin C antigen and antibody were diluted with PBSB (PBS containing 0.1% BSA) solution as blocking solution. The PBS buffer (pH 7.1) was used to covalently immobilize monoclonal antibodies on nanoparticles.