Cell death was measured using the Annexin V-FITC Apoptosis Detection Kit for flow cytometry (BD Biosciences #BDB556547). DLD1 cells were analyzed for cell death following KDSR KO at 8 and 10 days post lentiviral infection to induce CRISPR KO with CTRL nontargeting guide and 2 guides targeting KDSR. Briefly, cells were washed in PBS and stained with Annexin V-FITC and propidium iodide (PI) for 15 min protected from light. Cells were analyzed for the expression of Annexin V and PI immediately after staining by fluorescence associated cell sorting (FACS) using the BD LSR II flow cytometer. Each condition was infected and analyzed by flow cytometry in in biological triplicate. Flow cytometry data were analyzed using FlowJo Version 10, and the gating strategy and representative flow cytometry plots are shown in Figures S2D and S2E .
Annexin 5 fitc apoptosis detection kit for flow cytometry
Manufactured by BD
The Annexin V-FITC Apoptosis Detection Kit is a laboratory product used for flow cytometry analysis. It is designed to detect and quantify apoptosis, a form of programmed cell death, in cell samples. The kit contains Annexin V conjugated with the fluorescent dye FITC, which binds to phosphatidylserine, a marker of apoptotic cells. The kit also includes a propidium iodide solution for the identification of late apoptotic and necrotic cells.
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2 protocols using annexin 5 fitc apoptosis detection kit for flow cytometry
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Quantifying Apoptosis by Flow Cytometry
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Quantifying Apoptosis by Flow Cytometry
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Cell death was measured using the Annexin V-FITC Apoptosis Detection Kit for flow cytometry (BD Biosciences #BDB556547). DLD1 cells were analyzed for cell death following KDSR KO at 8 and 10 days post lentiviral infection to induce CRISPR KO with CTRL nontargeting guide and 2 guides targeting KDSR. Briefly, cells were washed in PBS and stained with Annexin V-FITC and propidium iodide (PI) for 15 min protected from light. Cells were analyzed for the expression of Annexin V and PI immediately after staining by fluorescence associated cell sorting (FACS) using the BD LSR II flow cytometer. Each condition was infected and analyzed by flow cytometry in in biological triplicate. Flow cytometry data were analyzed using FlowJo Version 10, and the gating strategy and representative flow cytometry plots are shown in Figures S2D and S2E .
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