Cell culture supernatant and serum samples were collected and stored at ‐80˚C. Protein levels of IL1β (MultiSciences, China), IL6 (MultiSciences, China), TNFα (Absin Bioscience, China), Cxcl1 (MultiSciences, China) and Cxcl2 (MultiSciences, China) were determined with specific ELISA kits according to the manufacturer's directions.
Tnf α
Manufactured by Absin
Sourced in China
TNF-α is a cytokine that plays a crucial role in the regulation of immune cells and inflammatory processes. It is commonly used in immunological research and drug discovery applications.
Automatically generated - may contain errors
Lab products found in correlation
Glutamate, by Merck Group (2 mentions)
Cytochalasin d, by Merck Group (2 mentions)
Taxol, by Merck Group (2 mentions)
Phospho stathmin 1 antibodies, by Cell Signaling Technology (2 mentions)
Stathmin antibodies, by Proteintech (2 mentions)
α tubulin antibodies, by Boster Bio (2 mentions)
Hrp labeled anti rabbit and anti mouse antibodies, by ZSGB-BIO (2 mentions)
Human vascular endothelial growth factor, by Thermo Fisher Scientific (2 mentions)
Capsaicin, by Absin (2 mentions)
Muscone, by Solarbio (2 mentions)
α asarone, by Solarbio (2 mentions)
Acrylamide, by Solarbio (2 mentions)
Hydrogen peroxide, by Merck Group (2 mentions)
Nocodazole, by Merck Group (2 mentions)
Histamine, by Thermo Fisher Scientific (2 mentions)
Jasplakinolide, by Merck Group (2 mentions)
β actin, by Boster Bio (2 mentions)
Lipopolysaccharides (lps), by Absin (2 mentions)
Cxcl1, by MultiSciences Biotech (1 mentions)
Il 1β, by MultiSciences Biotech (1 mentions)
5 protocols using tnf α
1
Cytokine Profiling in Cell Culture
2
Quantification of Inflammatory Markers
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3
Cytokine Profiling in MCMV-Infected Brains
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Cerebra samples isolated from PBS-perfused mock- and MCMV-infected brains were minced in PBS supplemented with protease inhibitors (Roche), then centrifuged at 5000g for 10 minutes, and supernatants were assayed for TNF-α (Absin), IFN-γ (Absin), IL-1β (Absin), or IL-18 (R&D Systems) following the manufacturer’s instructions.
4
Signaling Molecule Antibody Procurement
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Phospho-Cofilin and Phospho-stathmin-1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Cofilin and Stathmin antibodies were purchased from Proteintech (Chicago, USA). β-actin and α-tubulin antibodies were from Boster (Wuhan, China). HRP-labeled anti-rabbit and anti-mouse antibodies were purchased from Zsgb-Bio (Beijing, China).
Cytochalasin D and nocodazole were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Glutamate, hydrogen peroxide, jasplakinolide, and taxol were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Human vascular endothelial growth factor (VEGF) and histamine were obtained from Peprotech (Rocky Hill, NJ, USA). LPS, TNF-α, Ang-II, and Capsaicin were purchased from Absin (Shanghai, China). Acrylamide, Muscone, and α-Asarone were from Solarbio (Beijing, China).
Cytochalasin D and nocodazole were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Glutamate, hydrogen peroxide, jasplakinolide, and taxol were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Human vascular endothelial growth factor (VEGF) and histamine were obtained from Peprotech (Rocky Hill, NJ, USA). LPS, TNF-α, Ang-II, and Capsaicin were purchased from Absin (Shanghai, China). Acrylamide, Muscone, and α-Asarone were from Solarbio (Beijing, China).
5
Molecular Signaling Pathway Antibodies
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Phospho-co lin and Phospho-stathmin-1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Co lin and Stathmin antibodies were purchased form proteintech (Chicago, USA). βactin and α-tubulin antibodies were from Boster (Wuhan, China). HRP-labeled anti-rabbit and anti-mouse antibodies were purchased from Zsgb-Bio (Beijing, China).
Cytochalasin D and nocodazole were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Glutamate, hydrogen peroxide, jasplakinolide, and taxol were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Human vascular endothelial growth factor (VEGF) and histamine were obtained from Peprotech (Rocky Hill, NJ, USA). LPS, TNF-α, Ang-, and Capsaicin were purchased from Absin (Shanghai, China). Acrylamide, Muscone, and α-Asarone were from Solarbio (Beijing, China).
Cytochalasin D and nocodazole were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Glutamate, hydrogen peroxide, jasplakinolide, and taxol were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Human vascular endothelial growth factor (VEGF) and histamine were obtained from Peprotech (Rocky Hill, NJ, USA). LPS, TNF-α, Ang-, and Capsaicin were purchased from Absin (Shanghai, China). Acrylamide, Muscone, and α-Asarone were from Solarbio (Beijing, China).
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