Ftir thermo nicolet 380 spectrophotometer

For UV-Vis analysis, the measurements were performed at 1 nm resolution, and the wavelength range between 350 and 700 nm (Analytik Jena Specord 210 Plus190—UV-Vis-1100 nm, Germany). The samples were measured at the initial moment and after 1, 2, and 3 hours.
FTIR analysis involves mixing the AgNPs powder with KBr in agate mortar (1:100). The spectra for spruce bark extract and tested solutions were observed with FTIR Thermo Nicolet 380 Spectrophotometer (Thermo Scientific, Waltham, USA, range 4000–400 cm⁻¹).
The morphology and size of the synthesized AgNPs were characterized by transmission electron microscopy using a JEOL 100 U TEM microscope (Japan Electron Optics Laboratory, Tokyo, Japan) at 100 kV on nitrocellulose substrates. The surface-enhanced Raman spectroscopy was recorded with Raman Systems R3000 CN, equipped with a 785 nm diode. The laser power was 200 mV, and the integration time was 30 s. The images were processed with ImageJ program (Wayne Rasband, MD, USA).

For the FT-IR analysis, the AgNP dry matter was used. FT-IR analysis involves mixing the obtained powder with KBr in the agate mortar in a ratio of 1:100. Using the FT-IR Thermo Nicolet 380 Spectrophotometer (Thermo Scientific, Waltham, USA), the measuring range 4000–400 cm⁻¹, spectra were recorded for each TS.