Samples from all the diet components and feces were milled at 1 and 0.5 mm (Retsch cutting mill, model SM1, Retsch GmbH, Haan, Germany) and analyzed through AOAC [22 ] procedures for dry matter (DM) (934.01), organic matter (OM) (942.05), ether extract (EE) (920.39), crude protein (CP) (954.01), and starch (996.11). A Velp Scientifica apparatus (Velp Scientifica srl, Usmate, Italy) was used to determine Kjedhal N, which was then multiplied by a conversion factor of 6.25 to determine CP content. A solvent recovery extractor for oils and fats (JP Selecta, Det-Gras N, Barcelona, Spain) was used to determine EE content. To determine starch content, an enzymatic kit K-TSTA-100A (Megazyme, Enterprise Ireland, Dublin, Ireland) and a spectrometer UVmin-1240 (Shimadzu, Quioto, Japan) were used. Neutral detergent fiber (NDF) and acid detergent fiber (ADF) were obtained through the Robertson and Van Soest and Van Soest et al. [23 ,24 (link)] methodologies. Acid detergent lignin (ADL) was determined by solubilization of cellulose with sulfuric acid [23 ]. Soluble sugar content was determined using a UV-VIS spectrophotometry UVmin-1240 (Shimadzu, Quioto, Japan; 625 nm) through the anthrone methodology [25 (link)].
Uvmin 1240
Manufactured by Shimadzu
Sourced in Japan
The UVmin-1240 is a compact and lightweight UV-Vis spectrophotometer designed for routine analysis in various laboratory settings. It features a wavelength range of 190 to 1100 nm and can perform absorbance, transmittance, and concentration measurements.
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Lab products found in correlation
4 protocols using uvmin 1240
1
Comprehensive Nutritional Analysis of Dietary Components and Feces
2
Acorn Flour Starch Characterization
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Acorn flour starch content was determined using an enzymatic kit K-TSTA-100A (Megazyme, Enterprise Ireland, Dublin, Ireland), and then was measured in a spectrometer UVmin-1240 (Shimadzu, Kyoto, Japan).
Starch microstructural characterization was carried out with a scanning electron microscopy – SEM (Hitachi SEM TM 3030Plus, Tokyo, Japan). Samples were observed with 400–1500x magnification.
Starch microstructural characterization was carried out with a scanning electron microscopy – SEM (Hitachi SEM TM 3030Plus, Tokyo, Japan). Samples were observed with 400–1500x magnification.
3
Dispersibility of Fe2O3 and Fe2O3·H2O Particles
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Fe2O3 and Fe2O3·H2O were suspended at 10 w/w% into the various polymer solutions by a homogeniser (3000 rpm (50/s), 30-45 s; HM-300, Hsiangtai Machinery Industry Co., Ltd., New Taipei, Taiwan). The suspensions were then centrifuged for 5 min using a centrifuge at 5590×g of relative centrifugal force (RCF). Each supernatant was diluted to one-tenth with distilled water, and each transmittance (%) was determined at 500 nm by a spectrophotometer (UVmin-1240, Shimadzu Corporation, Kyoto, Japan). The dispersibility of Fe2O3 and Fe2O3·H2O particles was evaluated in terms of the index for dispersibility (IFD), as defined in the following equation: IFD=100 − transmittance. The RCF was calculated by the following equation: RCF (× g)=1118×R×N2×10-8, where R is the radius of the centrifuge (cm) and N is the rotational speed (rpm).
4
Barium Sulfur Turbidimetry Analysis
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Barium available sulfur turbidimetry was used to determine the total sulfur and available sulfur levels of the test sample (Su and Gelius, 2020 (link); Yue et al., 2021 (link); Zhang et al., 2021 (link); Zhao et al., 2021 (link), 2022 (link)). According to the standard curve and the spectrophotometric value, the content of sulfur in the sample is calculated, the spectrophotometer adopts UV-min1240 (Shimadzu).
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