Micro computerized tomography

Manufactured by Scanco

Sourced in Switzerland

Micro-computerized tomography is a non-destructive, high-resolution imaging technique that uses X-rays to create detailed three-dimensional models of small samples. It allows for the visualization and analysis of internal structures with a high degree of precision.

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Lab products found in correlation

Mimics software, by Materialise (3 mentions)

3 protocols using micro computerized tomography

Micro-CT Analysis of Cartilage and Bone

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Mineralized cartilage and subchondral bone was analyzed using micro-computerized tomography (SCANCO Medical AG, Brüttisellen, Switzerland). The samples were scanned in liquid, one at a time, with high resolution in a 16mm holder. Serial tomographic projections were acquired at 55kV and 145μA, with a voxel size of 6μm and 1000 projections per rotation collected at 300000μs. The DICOM images were transferred, segmented and reconstructed using the mimics software (Materialise, Belgium). In order to distinguish calcified tissue from non-calcified tissue, an automated algorithm using local threshold segmented the reconstructed grey scale images. Bone mineral density (BMD (mg/cc)), bone volume fraction (BVF (%)), trabecular thickness (Tb.Th (um)), and trabecular spacing (Tb.Sp (um)) were determined.

Kaul R., O’Brien M.H., Dutra E., Lima A., Utreja A, & Yadav S. (2016). The Effect of Altered Loading on Mandibular Condylar Cartilage. PLoS ONE, 11(7), e0160121.

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Botox Alters Cartilage and Bone

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Mineralized cartilage and subchondral bone in the control and Botox were analyzed using micro-computerized tomography (SCANCO Medical AG, Brüttisellen, Switzerland). The samples were scanned in 70% ethanol, one at a time, with high resolution in a 16mm holder. Serial tomographic projections were acquired at 55kV and 145μA, with a voxel size of 6μm and 1000 projections per rotation collected at 300000μs. The DICOM images were transferred, segmented and reconstructed using the mimics software (Materialise, Belgium). In order to distinguish calcified tissue from non-calcified tissue, an automated algorithm using local threshold segmented the reconstructed grey scale images. Bone mineral density (BMD (mg/cc)), bone volume fraction (BVF (%)), trabecular thickness (Tb.Th (μm)), and trabecular spacing (Tb.Sp (μm)) were determined.

Dutra E.H., O’ Brien M.H., Lima A., Kalajzic Z., Tadinada A., Nanda R, & Yadav S. (2016). Cellular and Matrix Response of the Mandibular Condylar Cartilage to Botulinum Toxin. PLoS ONE, 11(10), e0164599.

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Micro-CT Analysis of Articular Cartilage

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We used micro-computerized tomography (SCANCO Medical AG, Brüttisellen, Switzerland) to analyze the bone volume fraction (BVF (%)) of the mineralized cartilage and subchondral bone of condyles of experimental and control mice. For BVF, the whole condyle head represented 100%, and that included unmineralized and mineralized tissue. The samples were scanned in 70% ethanol, one at a time, with high resolution in a 16mm holder. Serial tomographic projections were acquired at 55kV and 145μA, with a voxel size of 6μm and 1000 projections per rotation collected at 300000μs. The DICOM images were transferred, segmented and reconstructed using the mimics software (Materialise, Belgium). In order to distinguish calcified tissue from non-calcified tissue, an automated algorithm using local threshold segmented the reconstructed grey scale images.

Dutra E.H, & Yadav S. (2019). The effects of Botox injection into the masseter in the mandibular condyle are not transient. American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics, 156(2), 193-202.

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