GelMA at a final concentration of 5% (w/v) was used as the main constituent of the bioink. DMSO, along with different saccharides listed in Table 1 , were added as the CPAs. D-(+)-trehalose dihydrate, D-lactose, sucrose ultrapure, D-(+) raffinose pentahydrate, and D-(+)-melezitose hydrate were purchased from Alfa Aesar. The saccharides’ concentration (0%, 4%, 8%, 12% (w/v)) and the DMSO concentration (0%, 5%, 10%, 15% (v/v)) were systematically studied to identify the optimal values based on cell viability results.
D lactose
Manufactured by Thermo Fisher Scientific
Sourced in Ireland
D-lactose is a monosaccharide that is widely used in the laboratory setting. It is the primary sugar found in mammalian milk and serves as an important energy source for many organisms. D-lactose is commonly utilized in various biochemical, microbiological, and cell culture applications.
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Lab products found in correlation
2 protocols using d lactose
1
Optimizing Cryoprotective Saccharides in GelMA Bioink
2
E. coli BL21 Growth Protocol
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All materials used in this manuscript have been previously described [9] with the exception of D-lactose (Alfa Aesar), glycerol (Sigma), and MILLEX-HV 0.22 μm Filter Unit (MILLIPORE, Carrigtwohil, Co. Cork, Ireland).
Cell Growth: All growths derived from E. coli BL21Star™(DE3) cells (generously provided by the Jewett Laboratory) are acquired from a glycerol stock and streaked onto a LB agar plate less than two weeks old and stored at 4°C. Streak plates were used within two weeks of inoculation.
Cell Growth: All growths derived from E. coli BL21Star™(DE3) cells (generously provided by the Jewett Laboratory) are acquired from a glycerol stock and streaked onto a LB agar plate less than two weeks old and stored at 4°C. Streak plates were used within two weeks of inoculation.
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