Penicillin was administered as a bath to oysters injected with pooled hemolymph from diseased oysters. For use in inoculation, pooled hemolymph from diseased pearl oysters collected from Shima (n = 5; a-value range 0.81–8.80; mean 5.16±2.58) and pooled hemolymph from healthy pearl oysters collected from Noto (negative control; n = 5; a-value range -0.43–2.51; mean 1.32±1.03) were injected into 80 and 40 oysters collected from Noto, respectively. Oysters injected with hemolymph from diseased oysters were divided into four experimental tanks with static water. Two of the tanks were treated with penicillin G potassium (Wako, Osaka, Japan) at a final concentration of 50 U/mL. The positive control tanks were filled with clean, unmedicated water. For the negative control, the two experimental tanks were filled with clean, unmedicated water. Water and penicillin were changed every 24 h, and penicillin was bath-administered for 10 consecutive days. In all tanks, water was kept at 25°C using an electric heater during penicillin administration, and seawater controlled at 23–25°C was flowed through the tanks after penicillin administration. Mortality was monitored for 111 days, and five individuals were randomly selected from among the survivors at 111 days to measure the a-value in adductor muscles.
Penicillin g potassium
Manufactured by Fujifilm
Sourced in Japan
Penicillin G potassium is a laboratory-grade antibiotic compound used for various applications in research and development. It is a salt form of the natural antibiotic penicillin G, which is derived from the Penicillium fungus. The compound is a white, crystalline powder that is soluble in water and other polar solvents. It is commonly used as a selective agent in cell culture media and for the prevention of bacterial contamination in various biological experiments.
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Lab products found in correlation
Streptomycin sulfate, by Fujifilm (2 mentions)
Dmem high glucose, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
L glutamine, by Fujifilm (1 mentions)
β mercaptoethanol, by Fujifilm (1 mentions)
Nahco3, by Fujifilm (1 mentions)
D glucose, by Merck Group (1 mentions)
Sodium bicarbonate, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
3 protocols using penicillin g potassium
1
Penicillin Treatment for Oyster Hemolymph Infection
2
3T3-L1 Preadipocyte Cell Culture
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3T3-L1 cells were obtained from the Health Science Research Resources Bank, Japanese Collection of Research Bioresources Cell Bank (JCRB9014), Osaka, Japan. 3T3-L1 preadipocytes were maintained in Dulbecco’s Modified Eagle Medium (DMEM, 5.5 mM glucose; Nissui Seiyaku, Tokyo, Japan) and supplemented with 1.5 g/L NaHCO3 (Wako, Osaka, Japan), 4.0 mM L-glutamine (Wako), 18 μg/mL penicillin G potassium (Meiji Seika, Tokyo, Japan), and 50 μg/mL streptomycin sulfate (Meiji Seika) in heat-inactivated 10% fetal bovine serum (FBS; Biowest, Miami, FL, USA) at 37°C in a humidified atmosphere containing 5% volume-percent (v/v) CO2. Cells were grown in 150 cm2 canted neck polystyrene flasks and were passaged at 80% confluency. When grown under the 25 mM glucose condition, DMEM high glucose (Sigma-Aldrich) was used and was supplemented with 1.7 g/L NaHCO3, 75 µg/mL penicillin G potassium, 50 µg/mL streptomycin sulfate, and 5 µL/L β-mercaptoethanol (Wako) in heat-inactivated 10% FBS.
3
αTC1.6 Cell Culture Maintenance
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αTC1.6 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). Cells were maintained in DMEM (Invitrogen, Carlsbad, CA, USA) containing 10% FCS (Thermo, Waltham, MA, USA), 15 mM HEPES (Gibco, Carlsbad, CA, USA), 0.1 mM non-essential amino acids (Gibco), 0.02% BSA (Sigma, St. Louis, MO, USA), 1.5 g/dL sodium bicarbonate (Sigma), 2 g/L d -glucose (Sigma), 75 mg/mL penicillin G potassium (Wako, Osaka, Japan), and 100 mg/L streptomycin sulfate (Wako) at 37 °C in a 5%-CO2 humidified incubator.
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