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2 protocols using s butyrylthiocholine iodide btci

1

Enzyme Assays for Neurodegenerative Disorders

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AChE (Type VI-S; from Electrophorus electricus), recombinant human MAO-A, MAO-B, and BChE (from equine serum), acetylthiocholine iodide (ATCI), kynuramine, benzylamine, S-butyrylthiocholine iodide (BTCI), 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), tacrine, donepezil, toloxatone, and lazabemide were purchased from Sigma-Aldrich (St. Louis, MO, USA). Clorgyline and pargyline (irreversible reference inhibitors of MAO-A and MAO-B, respectively) were from BioAssay Systems (Hayward, CA, USA)40 (link).
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2

Comprehensive Antioxidant Evaluation Protocol

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Folin–Ciocalteu reagent, DPPH (1, 1′-diphenyl-2-picrylhydrazyl), aluminum chloride, potassium ferricyanide, and ammonium molybdate were procured from Sigma-Aldrich, India. 2-Deoxy-D-ribose, 2-thiobarbituric acid (TBA), bromocresol green (BCG), catechin, quercetin, 5,5′-dithio-bis-(2-nitro) benzoic acid (DTNB), acetylthiocholine iodide (ATCI), and S-butyrylthiocholine iodide (BTCI) were obtained from Sigma-Aldrich, Germany. All the employed chemicals were of analytical grade.
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