Anti nk1.1 apc clone pk 136

Manufactured by BioLegend

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Anti-NK1.1-APC(clone PK 136) is a mouse monoclonal antibody conjugated with Allophycocyanin (APC) that specifically binds to the NK1.1 antigen expressed on natural killer cells in mice.

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Anti-NK1.1 (33 citations) NK1.1-APC (11 citations) Anti-NK1.1-APC (5 citations) APC-NK1.1 (4 citations)

3 protocols using anti nk1.1 apc clone pk 136

Comprehensive Immune Cell Profiling

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The following antibodies were used in a surface panel cocktail for the Bl-Eng2 peptide experiments: anti-CD45–Alexa Fluor 488 (clone 30-F11; Biolegend, catalog no. 103122), anti-major histocompatibility complex (MHC) class II tetramer–phycoerythrin (PE), anti-CD4–BUV395 (clone GK1.1; BD, catalog no. 563790), anti-CD8–peridinin chlorophyll protein (PerCP)-Cy5.5 (clone 53-6.7; Biolegend, catalog no. 100734), anti-CD44–BV650 (clone IM7; Biolegend, catalog no. 1033049), anti-CD11b–allophycocyanin (APC) (clone M1/70; Biolegend, catalog no. 101212), anti-CD11c–APC (clone N418; Biolegend, catalog no. 117310), anti-NK1.1–APC (clone PK136; Biolegend, catalog no. 108710), and anti-B220–APC (clone RA3-62B; Biolegend, catalog no. 103212).

Wüthrich M., Dobson H.E., Ledesma Taira C., Okaa U.J., dos Santos Dias L., Isidoro-Ayza M., Petrovsky N, & Klein B.S. (2021). Combination Adjuvants Enhance Recombinant Protein Vaccine Protection against Fungal Infection. mBio, 12(4), e02018-21.

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Multiparameter Flow Cytometry Panels

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The following antibodies (BioLegend, San Diego, CA, USA) were added to 100 µL aliquots of 10⁵ Fc-blocked splenocytes prepared as described above to make a final 1/600 dilution: anti-CD1d-PE(clone 1B1), anti-B220-FITC (clone RA3-6B2), anti-CD93-APC (clone AA4.1), anti-CD138-PE-Cy7 (clone 281-2), anti-GL7-PE (clone GL7), anti-Ly6G-Alexa488 (clone 1A8), anti-Ly6C-PE (clone HK 1.4), anti-CD4-FITC (clone GK 1.5), anti-CD8-PE (clone 53-6.7), anti-NK1.1-APC(clone PK 136), anti-TCRβ-PE-Cy7 (clone H57-597).

Radwanska M., Nguyen H.T, & Magez S. (2021). African Trypanosomosis Obliterates DTPa Vaccine-Induced Functional Memory So That Post-Treatment Bordetella pertussis Challenge Fails to Trigger a Protective Recall Response. Vaccines, 9(6), 603.

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Multiparameter Flow Cytometry Panels

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Magez S., Li Z., Nguyen H.T., Pinto Torres J.E., Van Wielendaele P., Radwanska M., Began J., Zoll S, & Sterckx Y.G. (2021). The History of Anti-Trypanosome Vaccine Development Shows That Highly Immunogenic and Exposed Pathogen-Derived Antigens Are Not Necessarily Good Target Candidates: Enolase and ISG75 as Examples. Pathogens, 10(8), 1050.

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