The spheroids were fixed with 4% paraformaldehyde for 20 min, and after 30 min of incubation with the blocking solution (Triton 100 × 1:1000, 10% goat serum in PBS), they were incubated for 3 h with primary anti-Ki-67 antibody (1:150 dilution; Millipore) at 37 °C. The secondary FITC-conjugated goat anti-rabbit antibody (1:250 dilution; Millipore) was administered for 2 h and, after the washing steps, staining with TRITC-phalloidin (1:200 dilution; Sigma-Aldrich) and Hoechst 33342 (1:1000 dilution; Invitrogen) in PBS was carried out for 1 h at 37 °C. Images were acquired with a C2s confocal microscope (Nikon).
Primary anti ki 67 antibody
Manufactured by Merck Group
The Primary anti-Ki-67 antibody is a laboratory reagent used for the detection of the Ki-67 protein, which is a marker of cellular proliferation. This antibody is used in various research and diagnostic applications, such as immunohistochemistry and flow cytometry, to identify and quantify proliferating cells.
Automatically generated - may contain errors
2 protocols using primary anti ki 67 antibody
1
Immunohistochemistry of Ki-67 in Spheroids
2
Immunohistochemistry of Ki-67 in Spheroids
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The spheroids were fixed with 4% paraformaldehyde for 20 min, and after 30 min of incubation with the blocking solution (Triton 100 × 1:1000, 10% goat serum in PBS), they were incubated for 3 h with primary anti-Ki-67 antibody (1:150 dilution; Millipore) at 37 °C. The secondary FITC-conjugated goat anti-rabbit antibody (1:250 dilution; Millipore) was administered for 2 h and, after the washing steps, staining with TRITC-phalloidin (1:200 dilution; Sigma-Aldrich) and Hoechst 33342 (1:1000 dilution; Invitrogen) in PBS was carried out for 1 h at 37 °C. Images were acquired with a C2s confocal microscope (Nikon).
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